Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153734
Title: A fluorescence kinetic-based aptasensor employing stilbene isomerization for detection of thrombin
Authors: Zeng, Xinling
Zhou, Qing
Wang, Liyan
Zhu, Xiaoxian
Cui, Kuiyan
Peng, Xinsheng
Steele, Terry W. J.
Chen, Huizhi
Xu, Hui
Zhou, Yubin
Keywords: Engineering::Materials
Issue Date: 2021
Source: Zeng, X., Zhou, Q., Wang, L., Zhu, X., Cui, K., Peng, X., Steele, T. W. J., Chen, H., Xu, H. & Zhou, Y. (2021). A fluorescence kinetic-based aptasensor employing stilbene isomerization for detection of thrombin. Materials, 14(22), 6927-. https://dx.doi.org/10.3390/ma14226927
Journal: Materials
Abstract: It is important to detect thrombin due to its physiological and pathological roles, where rapid and simple analytical approaches are needed. In this study, an aptasensor based on fluorescence attenuation kinetics for the detection of thrombin is presented, which incorporates the features of stilbene and aptamer. We designed and synthesized an aptasensor by one-step coupling of stilbene compound and aptamer, which employed the adaptive binding of the aptamer with thrombin to cause a change in stilbene fluorescence attenuation kinetics. The sensor realized detection of thrombin by monitoring the variation in apparent fluorescence attenuation rate constant (kₐₚₚ), which could be further used for probing of enzyme-aptamer binding. In comprehensive studies, the developed aptasensor presented satisfactory performance on repeatability, specificity, and regeneration capacity, which realized rapid sensing (10 s) with a limit of detection (LOD) of 0.205 μM. The strategy was successful across seven variants of thrombin aptasensors, with tunable kapp depending on the SITS (4-Acetamido-4'-isothiocyanato-2,2'-stilbenedisulfonic acid disodium salt hydrate) grafting site. Analyte detection mode was demonstrated in diluted serum, requiring no separation or washing steps. The new sensing mode for thrombin detection paves a way for high-throughput kinetic-based sensors for exploiting aptamers targeted at clinically relevant proteins.
URI: https://hdl.handle.net/10356/153734
ISSN: 1996-1944
DOI: 10.3390/ma14226927
Rights: © 2021 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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