Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153763
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBenninghoff, Jens C.en_US
dc.contributor.authorKuschmierz, Lauraen_US
dc.contributor.authorZhou, Xiaoxiaoen_US
dc.contributor.authorAlbersmeier, Andreasen_US
dc.contributor.authorPham, Trong Khoaen_US
dc.contributor.authorBusche, Tobiasen_US
dc.contributor.authorWright, Phillip C.en_US
dc.contributor.authorKalinowski, Jornen_US
dc.contributor.authorMakarova, Kira S.en_US
dc.contributor.authorBrasen, Christopheren_US
dc.contributor.authorFlemming, Hans-Curten_US
dc.contributor.authorWingender, Josten_US
dc.contributor.authorSiebers, Bettinaen_US
dc.date.accessioned2021-12-16T06:34:28Z-
dc.date.available2021-12-16T06:34:28Z-
dc.date.issued2021-
dc.identifier.citationBenninghoff, J. C., Kuschmierz, L., Zhou, X., Albersmeier, A., Pham, T. K., Busche, T., Wright, P. C., Kalinowski, J., Makarova, K. S., Brasen, C., Flemming, H., Wingender, J. & Siebers, B. (2021). Exposure to 1-butanol exemplifies the response of the thermoacidophilic archaeon sulfolobus acidocaldarius to solvent stress. Applied and Environmental Microbiology, 87(11), e02988-20-. https://dx.doi.org/10.1128/AEM.02988-20en_US
dc.identifier.issn0099-2240en_US
dc.identifier.urihttps://hdl.handle.net/10356/153763-
dc.description.abstractSulfolobus acidocaldarius is a thermoacidophilic crenarchaeon with optimal growth at 80 degrees C and pH 2 to 3. Due to its unique physiological properties, allowing life at environmental extremes, and the recent availability of genetic tools, this extremophile has received increasing interest for biotechnological applications. In order to elucidate the potential of tolerating process-related stress conditions, we investigated the response of S. acidocaldarius toward the industrially relevant organic solvent 1-butanol. In response to butanol exposure, biofilm formation of S. acidocaldarius was enhanced and occurred at up to 1.5% (vol/vol) 1-butanol, while planktonic growth was observed at up to 1% (vol/vol) 1-butanol. Confocal laser-scanning microscopy revealed that biofilm architecture changed with the formation of denser and higher tower-like structures. Concomitantly, changes in the extracellular polymeric substances with enhanced carbohydrate and protein content were determined in 1-butanol-exposed biofilms. Using scanning electron microscopy, three different cell morphotypes were observed in response to 1-butanol. Transcriptome and proteome analyses were performed comparing the response of planktonic and biofilm cells in the absence and presence of 1-butanol. In response to 1% (vol/vol) 1-butanol, transcript levels of genes encoding motility and cell envelope structures, as well as membrane proteins, were reduced. Cell division and/or vesicle formation were upregulated. Furthermore, changes in immune and defense systems, as well as metabolism and general stress responses, were observed. Our findings show that the extreme lifestyle of S. acidocaldarius coincided with a high tolerance to organic solvents. This study provides what may be the first insights into biofilm formation and membrane/cell stress caused by organic solvents in S. acidocaldarius. IMPORTANCE Archaea are unique in terms of metabolic and cellular processes, as well as the adaptation to extreme environments. In the past few years, the development of genetic systems and biochemical, genetic, and polyomics studies has provided deep insights into the physiology of some archaeal model organisms. In this study, we used S. acidocaldarius, which is adapted to the two extremes of low pH and high temperature, to study its tolerance and robustness as well as its global cellular response toward organic solvents, as exemplified by 1-butanol. We were able to identify biofilm formation as a primary cellular response to 1-butanol. Furthermore, the triggered cell/membrane stress led to significant changes in culture heterogeneity accompanied by changes in central cellular processes, such as cell division and cellular defense systems, thus suggesting a global response for the protection at the population level.en_US
dc.language.isoenen_US
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.rights© 2021 Benninghoff et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.en_US
dc.subjectEngineering::Environmental engineeringen_US
dc.titleExposure to 1-butanol exemplifies the response of the thermoacidophilic archaeon sulfolobus acidocaldarius to solvent stressen_US
dc.typeJournal Articleen
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineeringen_US
dc.identifier.doi10.1128/AEM.02988-20-
dc.description.versionPublished versionen_US
dc.identifier.issue11en_US
dc.identifier.volume87en_US
dc.identifier.spagee02988-20en_US
dc.subject.keywordsArchaeaen_US
dc.subject.keywordsExtremophilesen_US
dc.description.acknowledgement.C.B. acknowledges funding by the Mercator foundation for support with a Mercur startup grant (Pr-2011-0058) and by the German Federal Ministry of Education and Research (BMBF) grant SulfoSYSBIOTECH, 0316188A within the e:Bio funding initiative. A.A. was supported by grant 0316188D within SulfoSYSBIOTECH. L.K. acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, SI 642/13-1). X.Z. acknowledges funding by the VW Stiftung in the “Life?” initiative (96725). K.S.M. is supported by the Intramural Research Program of the National Institutes of Health of the USA (National Library of Medicine). T.K.P. and P.C.W. acknowledge the BBSRC funding BB/M018172/1 and BB/M018288/1.en_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SCELSE Journal Articles
Files in This Item:
File Description SizeFormat 
AEM.02988-20.pdf4.01 MBAdobe PDFThumbnail
View/Open

Page view(s)

32
Updated on Jul 5, 2022

Download(s)

7
Updated on Jul 5, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.