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|Title:||Cx43 mediates changes in myofibroblast contraction and collagen release in human amniotic membrane defects after trauma||Authors:||Costa, Eleni
Okesola, Babatunde O.
Becker, David Lawrence
Deprest, Jan A.
David, Anna L.
Chowdhury, Tina T.
|Keywords:||Science::Medicine||Issue Date:||2021||Source:||Costa, E., Okesola, B. O., Thrasivoulou, C., Becker, D. L., Deprest, J. A., David, A. L. & Chowdhury, T. T. (2021). Cx43 mediates changes in myofibroblast contraction and collagen release in human amniotic membrane defects after trauma. Scientific Reports, 11(1), 16975-. https://dx.doi.org/10.1038/s41598-021-94767-4||Journal:||Scientific Reports||Abstract:||The wound healing capacity of the fetal membranes after spontaneous or iatrogenic membrane rupture is unclear. We examined the healing mechanisms in amniotic membrane (AM) defects after trauma. Traumatised human AM defects were cultured for 4 days. Markers for nuclear (DAPI), cell type (vimentin, αSMA) and healing (Cx43, TGFβ1, collagen) were examined by immunofluorescence (IMF) confocal microscopy, Second Harmonic Generation (SHG) imaging and RT-qPCR. After trauma, AMCs and myofibroblasts migrated to the AM wound edge. Within four days, αSMA expressing myofibroblasts showed abundant Cx43 localized in the cytoplasmic processes. The highly contractile spindle-shaped myofibroblasts were present in the defect site and released collagen. In contrast, AMCs expressed vimentin and formed Cx43 plaques between cells found in the outer edges of the wound. Whilst AMCs were absent in the defect site, αSMA expressing myofibroblasts continued to elongate and polarize the collagen fibres. Both TGFβ1 and Cx43 gene expression were significantly increased after trauma. Cx43 has differential effects on AM cell populations that increase cellularity, contraction and potentially migration to the wound edge resulting in collagen polarisation in the AM defect site. Establishing how Cx43 regulates AM cell function could be an approach to repair defects in the membranes after trauma.||URI:||https://hdl.handle.net/10356/153774||ISSN:||2045-2322||DOI:||10.1038/s41598-021-94767-4||Rights:||© 2021 The Author(s). Tis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||LKCMedicine Journal Articles|
Updated on Jan 17, 2022
Updated on Jan 17, 2022
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