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Title: Ocular delivery of predatory bacteria with cryomicroneedles against eye infection
Authors: Cui, Mingyue
Zheng, Mengjia
Wiraja, Christian
Chew, Sharon Wan Ting
Mishra, Arti
Mayandi, Venkatesh
Lakshminarayanan, Rajamani
Xu, Chenjie
Keywords: Engineering::Chemical engineering
Issue Date: 2021
Source: Cui, M., Zheng, M., Wiraja, C., Chew, S. W. T., Mishra, A., Mayandi, V., Lakshminarayanan, R. & Xu, C. (2021). Ocular delivery of predatory bacteria with cryomicroneedles against eye infection. Advanced Science, 8(21), 2102327-.
Project: SHF/FG663P/2017
Journal: Advanced Science 
Abstract: The development of potent antibiotic alternatives with rapid bactericidal properties is of great importance in addressing the current antibiotic crisis. One representative example is the topical delivery of predatory bacteria to treat ocular bacterial infections. However, there is a lack of suitable methods for the delivery of predatory bacteria into ocular tissue. This work introduces cryomicroneedles (cryoMN) for the ocular delivery of predatory Bdellovibrio bacteriovorus (B. bacteriovorus) bacteria. The cryoMN patches are prepared by freezing B. bacteriovorus containing a cryoprotectant medium in a microneedle template. The viability of B. bacteriovorus in cryoMNs remains above 80% as found in long-term storage studies, and they successfully impede the growth of gram-negative bacteria in vitro or in a rodent eye infection model. The infection is significantly relieved by nearly six times through 2.5 days of treatment without substantial effects on the cornea thickness and morphology. This approach represents the safe and efficient delivery of new class of antimicrobial armamentarium to otherwise impermeable ocular surface and opens up new avenues for the treatment of ocular surface disorders.
ISSN: 2198-3844
DOI: 10.1002/advs.202102327
Rights: © 2021 The Authors. Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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