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Title: Resistance of SARS-CoV-2 variants to neutralization by convalescent plasma from early COVID-19 outbreak in Singapore
Authors: Wang, Bei
Goh, Yun Shan
Prince, Tessa
Ngoh, Eve Zi Xian
Salleh, Siti Nazihah Mohd
Hor, Pei Xiang
Loh, Chiew Yee
Fong, Siew Wai
Hartley, Catherine
Tan, Seow-Yen
Young, Barnaby Edward
Leo, Yee Sin
Lye, David Chien Boon
Maurer-Stroh, Sebastian
Ng, Lisa F. P.
Hiscox, Julian A.
Renia, Laurent
Wang, Cheng-I
Keywords: Science::Medicine
Issue Date: 2021
Source: Wang, B., Goh, Y. S., Prince, T., Ngoh, E. Z. X., Salleh, S. N. M., Hor, P. X., Loh, C. Y., Fong, S. W., Hartley, C., Tan, S., Young, B. E., Leo, Y. S., Lye, D. C. B., Maurer-Stroh, S., Ng, L. F. P., Hiscox, J. A., Renia, L. & Wang, C. (2021). Resistance of SARS-CoV-2 variants to neutralization by convalescent plasma from early COVID-19 outbreak in Singapore. Npj Vaccines, 6(1), 125-.
Project: ACCL/19-GAP064-R20H-H
Journal: npj Vaccines
Abstract: The rapid spreading of SARS-CoV-2 variants B.1.1.7 originated from the United Kingdom and B.1.351 from South Africa has contributed to the second wave of COVID-19 cases in the respective countries and also around the world. In this study, we employed advanced biochemical and virological methodologies to evaluate the impact of Spike mutations of these strains on the degree of protection afforded by humoral immune responses following natural infection of the ancestral SARS-CoV-2 strain during the early stages of the outbreak. We found that antibody-mediated neutralization activity was partially reduced for B.1.1.7 variant and significantly attenuated for the B.1.351 strain. We also found that mutations outside the receptor-binding domain (RBD) can strongly influence antibody binding and neutralization, cautioning the use of solely RBD mutations in evaluating vaccine efficacy. These findings highlight an urgent need to develop new SARS-CoV-2 vaccines that are not based exclusively on the ancestral SARS-CoV-2 Spike gene sequence.
ISSN: 2059-0105
DOI: 10.1038/s41541-021-00389-2
Rights: © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
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