Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153891
Title: Anti-fungal hevein-like peptides biosynthesized from quinoa cleavable hololectins
Authors: Loo, Shining
Tay, Stephanie Victoria
Kam, Antony
Tang, Fan
Fan, Jing-Song
Yang, Daiwen
Tam, James P.
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Loo, S., Tay, S. V., Kam, A., Tang, F., Fan, J., Yang, D. & Tam, J. P. (2021). Anti-fungal hevein-like peptides biosynthesized from quinoa cleavable hololectins. Molecules, 26(19), 5909-. https://dx.doi.org/10.3390/molecules26195909
Project: MOE2016-T3-1-003
Journal: Molecules 
Abstract: Chitin-binding hevein-like peptides (CB-HLPs) belong to a family of cysteine-rich peptides that play important roles in plant stress and defense mechanisms. CB-HLPs are ribosomally synthesized peptides that are known to be bioprocessed from the following two types of three-domain CB-HLP precursor architectures: cargo-carrying and non-cargo-carrying. Here, we report the identification and characterization of chenotides biosynthesized from the third type of precursors, which are cleavable hololectins of the quinoa (Chenopodium quinoa) family. Chenotides are 6-Cys-CB-HLPs of 29-31 amino acids, which have a third type of precursor architecture that encompasses a canonical chitin-binding domain that is involved in chitin binding and anti-fungal activities. Microbroth dilution assays and microscopic analyses showed that chenotides are effective against phyto-pathogenic fungi in the micromolar range. Structure determination revealed that chenotides are cystine knotted and highly compact, which could confer resistance against heat and proteolytic degradation. Importantly, chenotides are connected by a novel 18-residue Gly/Ala-rich linker that is a target for bioprocessing by cathepsin-like endopeptidases. Taken together, our findings reveal that chenotides are a new family of CB-HLPs from quinoa that are synthesized as a single multi-modular unit and bioprocessed to yield individual mature CB-HLPs. Importantly, such precursors constitute a new family of cleavable hololectins. This unusual feature could increase the biosynthetic efficiency of anti-fungal CB-HLPs, to provide an evolutionary advantage for plant survival and reproduction.
URI: https://hdl.handle.net/10356/153891
ISSN: 1420-3049
DOI: 10.3390/molecules26195909
Rights: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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