Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/153894
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dc.contributor.authorHumaidi, Mahathiren_US
dc.contributor.authorTien, Wei Pingen_US
dc.contributor.authorYap, Graceen_US
dc.contributor.authorChua, Choon Rongen_US
dc.contributor.authorNg, Lee-Chingen_US
dc.date.accessioned2021-12-30T07:55:59Z-
dc.date.available2021-12-30T07:55:59Z-
dc.date.issued2021-
dc.identifier.citationHumaidi, M., Tien, W. P., Yap, G., Chua, C. R. & Ng, L. (2021). Non-invasive dengue diagnostics - the use of saliva and urine for different stages of the illness. Diagnostics, 11(8), 1345-. https://dx.doi.org/10.3390/diagnostics11081345en_US
dc.identifier.issn2075-4418en_US
dc.identifier.urihttps://hdl.handle.net/10356/153894-
dc.description.abstractDengue diagnosis is largely dependent on clinical symptoms and routinely confirmed with laboratory detection of dengue virus in patient serum samples collected via phlebotomy. This presents a challenge to patients not amenable to venipuncture. Non-invasive methods of dengue diagnosis have the potential to enhance the current dengue detection algorithm. In this study, samples from dengue infected patients were collected between January 2012 until September 2012 and September 2013 until December 2013 in two different setups. Panel A samples (blood, urine, and saliva) were collected daily when the 39 patients were hospitalised and during their follow-up visits while Panel B samples (saliva) were collected from 23 patients during the acute stage of dengue. Using DENV PCR on Panel A, from day 2 to day 4 post fever onset, serum showed the best overall positivity followed by saliva and urine (100%/82.1%/67.9%). From day 5 until day 10 post fever onset, serum and urine had similar positivity (67.4%/61.2%), followed by saliva (51.3%). Beyond day 10 post fever onset, DENV was undetectable in sera, but urine and saliva showed 56.8% and 28.6% positivity, respectively. DENV in urine was detectable up until 32 days post fever. Panel B results showed overall sensitivity of 32.4%/36% (RNA/NS1) for DENV detection in saliva. Our results suggest that the urine-based detection method is useful especially for late dengue detection, where DENV is undetected in sera but still detectable in urine. This provides a potential tool for the physician to pick up new cases in an area where there is ongoing dengue transmission and subsequently prompt for intensified vector control activities.en_US
dc.language.isoenen_US
dc.relation.ispartofDiagnosticsen_US
dc.rights© 2021 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).en_US
dc.subjectScience::Biological sciencesen_US
dc.titleNon-invasive dengue diagnostics - the use of saliva and urine for different stages of the illnessen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationEnvironmental Health Instituteen_US
dc.contributor.organizationNational Environment Agencyen_US
dc.identifier.doi10.3390/diagnostics11081345-
dc.description.versionPublished versionen_US
dc.identifier.pmid34441280-
dc.identifier.scopus2-s2.0-85111648913-
dc.identifier.issue8en_US
dc.identifier.volume11en_US
dc.identifier.spage1345en_US
dc.subject.keywordsDengueen_US
dc.subject.keywordsDiagnosticsen_US
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