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Title: Rodent malaria erythrocyte preference assessment by an ex vivo tropism assay
Authors: Leong, Yew Wai
Lee, Erica Qian Hui
Rénia, Laurent
Malleret, Benoit
Keywords: Science::Medicine
Issue Date: 2021
Source: Leong, Y. W., Lee, E. Q. H., Rénia, L. & Malleret, B. (2021). Rodent malaria erythrocyte preference assessment by an ex vivo tropism assay. Frontiers in Cellular and Infection Microbiology, 11, 680136-.
Project: NUHSRO/2018/006/SU/01
Journal: Frontiers in Cellular and Infection Microbiology 
Abstract: Circulating red blood cells consist of young erythrocytes (early and late reticulocytes) and mature erythrocytes (normocytes). The human malaria parasites, Plasmodium falciparum and P. vivax, have a preference to invade reticulocytes during blood-stage infection. Rodent malaria parasites that also prefer reticulocytes could be useful tools to study human malaria reticulocyte invasion. However, previous tropism studies of rodent malaria are inconsistent from one another, making it difficult to compare cell preference of different parasite species and strains. In vivo measurements of cell tropism are also subjected to many confounding factors. Here we developed an ex vivo tropism assay for rodent malaria with highly purified fractions of murine reticulocytes and normocytes. We measured invasion into the different erythrocyte populations using flow cytometry and evaluated the tropism index of the parasite strains. We found that P. berghei ANKA displayed the strongest reticulocyte preference, followed by P. yoelii 17X1.1, whereas P. chabaudi AS and P. vinckei S67 showed mixed tropism. These preferences are intrinsic and were maintained at different reticulocyte and normocyte availabilities. Our study shed light on the true erythrocyte preference of the parasites and paves the way for future investigations on the receptor-ligand interactions mediating erythrocyte tropism.
ISSN: 2235-2988
DOI: 10.3389/fcimb.2021.680136
Rights: © 2021 Leong, Lee, Renia and Malleret. This is an open-access article ́ distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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