Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/154000
Title: Ferroptosis induces membrane blebbing in placental trophoblasts
Authors: Kajiwara, Kazuhiro
Beharier, Ofer
Chng, Choon-Peng
Goff, Julie P.
Ouyang, Yingshi
St Croix, Claudette M.
Huang, Changjin
Kagan, Valerian E.
Hsia, K. Jimmy
Sadovsky, Yoel
Keywords: Engineering::Chemical engineering
Issue Date: 2022
Source: Kajiwara, K., Beharier, O., Chng, C., Goff, J. P., Ouyang, Y., St Croix, C. M., Huang, C., Kagan, V. E., Hsia, K. J. & Sadovsky, Y. (2022). Ferroptosis induces membrane blebbing in placental trophoblasts. Journal of Cell Science, 135(5), jcs255737-. https://dx.doi.org/10.1242/jcs.255737
Project: M4082428.050
M4082352.050
M4012229.050
Journal: Journal of Cell Science
Abstract: Ferroptosis is a regulated, non-apoptotic form of cell death, characterized by hydroxy-peroxidation of discrete phospholipid hydroperoxides, particularly hydroperoxyl (Hp) forms of arachidonoyl- and adrenoyl-phosphatidylethanolamine, with a downstream cascade of oxidative damage to membrane lipids, proteins and DNA, culminating in cell death. We recently showed that human trophoblasts are particularly sensitive to ferroptosis caused by depletion or inhibition of glutathione peroxidase 4 (GPX4) or the lipase PLA2G6. Here, we show that trophoblastic ferroptosis is accompanied by a dramatic change in the trophoblast plasma membrane, with macro-blebbing and vesiculation. Immunofluorescence revealed that ferroptotic cell-derived blebs stained positive for F-actin, but negative for cytoplasmic organelle markers. Transfer of conditioned medium that contained detached macrovesicles or co-culture of wild-type target cells with blebbing cells did not stimulate ferroptosis in target cells. Molecular modeling showed that the presence of Hp-phosphatidylethanolamine in the cell membrane promoted its cell ability to be stretched. Together, our data establish that membrane macro-blebbing is characteristic of trophoblast ferroptosis and can serve as a useful marker of this process. Whether or not these blebs are physiologically functional remains to be established.
URI: https://hdl.handle.net/10356/154000
ISSN: 0021-9533
DOI: 10.1242/jcs.255737
Rights: © 2021 The Author(s). All rights reserved. This paper was published by The Company of Biologists Ltd in Journal of Cell Science and is made available with permission of The Author(s).
Fulltext Permission: embargo_20230331
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles
SCBE Journal Articles

Files in This Item:
File Description SizeFormat 
jcs255737.pdf
  Until 2023-03-31
13.72 MBAdobe PDFUnder embargo until Mar 31, 2023

SCOPUSTM   
Citations 20

11
Updated on Nov 30, 2022

Web of ScienceTM
Citations 20

12
Updated on Dec 1, 2022

Page view(s)

22
Updated on Dec 1, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.