Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/154019
Title: Complement factor B mediates ocular angiogenesis through regulating the VEGF signaling pathway
Authors: Murray, Hannah
Qiu, Beiying
Ho, Sze Yuan
Wang, Xiaomeng
Keywords: Science::Medicine
Issue Date: 2021
Source: Murray, H., Qiu, B., Ho, S. Y. & Wang, X. (2021). Complement factor B mediates ocular angiogenesis through regulating the VEGF signaling pathway. International Journal of Molecular Sciences, 22(17), 9580-. https://dx.doi.org/10.3390/ijms22179580
Project: 2017T1-001-099
DYNAMO NMRC/OFLCG/001/2017
TAAP NMRC/OFLCG/004/2018
Journal: International Journal of Molecular Sciences
Abstract: Complement factor B (CFB), a 95-kDa protein, is a crucial catalytic element of the alternative pathway (AP) of complement. After binding of CFB to C3b, activation of the AP depends on the proteolytic cleavage of CFB by factor D to generate the C3 convertase (C3bBb). The C3 convertase contains the catalytic subunit of CFB (Bb), the enzymatic site for the cleavage of a new molecule of C3 into C3b. In addition to its role in activating the AP, CFB has been implicated in pathological ocular neovascularization, a common feature of several blinding eye diseases, however, with somewhat conflicting results. The focus of this study was to investigate the direct impact of CFB on ocular neovascularization in a tightly controlled environment. Using mouse models of laser-induced choroidal neovascularization (CNV) and oxygen-induced retinopathy (OIR), our study demonstrated an increase in CFB expression during pathological angiogenesis. Results from several in vitro and ex vivo functionality assays indicated a promoting effect of CFB in angiogenesis. Mechanistically, CFB exerts this pro-angiogenic effect by mediating the vascular endothelial growth factor (VEGF) signaling pathway. In summary, we demonstrate compelling evidence for the role of CFB in driving ocular angiogenesis in a VEGF-dependent manner. This work provides a framework for a more in-depth exploration of CFB-mediated effects in ocular angiogenesis in the future.
URI: https://hdl.handle.net/10356/154019
ISSN: 1661-6596
DOI: 10.3390/ijms22179580
Rights: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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