Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/154070
Title: Kv1.3 channel up-regulation in peripheral blood T lymphocytes of patients with multiple sclerosis
Authors: Markakis, Ioannis
Charitakis, Ioannis
Beeton, Christine
Galani, Melpomeni
Repousi, Elpida
Aggeloglou, Stella
Sfikakis, Petros P.
Pennington, Michael W.
Chandy, Kanianthara George
Poulopoulou, Cornelia
Keywords: Science::Medicine
Issue Date: 2021
Source: Markakis, I., Charitakis, I., Beeton, C., Galani, M., Repousi, E., Aggeloglou, S., Sfikakis, P. P., Pennington, M. W., Chandy, K. G. & Poulopoulou, C. (2021). Kv1.3 channel up-regulation in peripheral blood T lymphocytes of patients with multiple sclerosis. Frontiers in Pharmacology, 12, 714841-. https://dx.doi.org/10.3389/fphar.2021.714841
Journal: Frontiers in Pharmacology
Abstract: Voltage-gated Kv1.3 potassium channels are key regulators of T lymphocyte activation, proliferation and cytokine production, by providing the necessary membrane hyper-polarization for calcium influx following immune stimulation. It is noteworthy that an accumulating body of in vivo and in vitro evidence links these channels to multiple sclerosis pathophysiology. Here we studied the electrophysiological properties and the transcriptional and translational expression of T lymphocyte Kv1.3 channels in multiple sclerosis, by combining patch clamp recordings, reverse transcription polymerase chain reaction and flow cytometry on freshly isolated peripheral blood T lymphocytes from two patient cohorts with multiple sclerosis, as well as from healthy and disease controls. Our data demonstrate that T lymphocytes in MS, manifest a significant up-regulation of Kv1.3 mRNA, Kv1.3 membrane protein and Kv1.3 current density and therefore of functional membrane channel protein, compared to control groups (p < 0.001). Interestingly, patient sub-grouping shows that Kv1.3 channel density is significantly higher in secondary progressive, compared to relapsing-remitting multiple sclerosis (p < 0.001). Taking into account the tight connection between Kv1.3 channel activity and calcium-dependent processes, our data predict and could partly explain the reported alterations of T lymphocyte function in multiple sclerosis, while they highlight Kv1.3 channels as potential therapeutic targets and peripheral biomarkers for the disease.
URI: https://hdl.handle.net/10356/154070
ISSN: 1663-9812
DOI: 10.3389/fphar.2021.714841
Rights: © 2021 Markakis, Charitakis, Beeton, Galani, Repousi, Aggeloglou, Sfikakis, Pennington, Chandy and Poulopoulou. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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