Please use this identifier to cite or link to this item:
Title: Precisely structured nitric-oxide-releasing copolymer brush defeats broad-spectrum catheter-associated biofilm infections in vivo
Authors: Hou, Zheng
Wu, Yang
Xu, Chen
Reghu, Sheethal
Shang, Zifang
Chen, Jingjie
Pranantyo, Dicky
Marimuth, Kalisvar
De, Partha Pratim
Ng, Oon Tek
Pethe, Kevin
Kang, En-Tang
Li, Peng
Chan-Park, Mary B.
Keywords: Engineering::Chemical engineering::Polymers and polymer manufacture
Issue Date: 2020
Source: Hou, Z., Wu, Y., Xu, C., Reghu, S., Shang, Z., Chen, J., Pranantyo, D., Marimuth, K., De, P. P., Ng, O. T., Pethe, K., Kang, E., Li, P. & Chan-Park, M. B. (2020). Precisely structured nitric-oxide-releasing copolymer brush defeats broad-spectrum catheter-associated biofilm infections in vivo. ACS Central Science, 6(11), 2031-2045.
Journal: ACS Central Science
Abstract: Gram-negative bacteria cannot be easily eradicated by antibiotics and are a major source of recalcitrant infections of indwelling medical devices. Among various device-associated infections, intravascular catheter infection is a leading cause of mortality. Prior approaches to surface modification, such as antibiotics impregnation, hydrophilization, unstructured NO-releasing, etc., have failed to achieve adequate infection-resistant coatings. We report a precision-structured diblock copolymer brush (H(N)-b-S) composed of a surface antifouling block of poly(sulfobetaine methacrylate) (S) and a subsurface bactericidal block (H(N)) of nitric-oxide-emitting functionalized poly(hydroxyethyl methacrylate) (H) covalently grafted from the inner and outer surfaces of a polyurethane catheter. The block copolymer architecture of the coating is important for achieving good broad-spectrum anti-biofilm activity with good biocompatibility and low fouling. The coating procedure is scalable to clinically useful catheter lengths. Only the block copolymer brush coating ((H(N)-b-S)) shows unprecedented, above 99.99%, in vitro biofilm inhibition of Gram-positive and Gram-negative bacteria, 100-fold better than previous coatings. It has negligible toxicity toward mammalian cells and excellent blood compatibility. In a murine subcutaneous infection model, it achieves >99.99% biofilm reduction of Gram-positive and Gram-negative bacteria compared with <90% for silver catheter, while in a porcine central venous catheter infection model, it achieves >99.99% reduction of MRSA with 5-day implantation. This precision coating is readily applicable for long-term biofilm-resistant and blood-compatible copolymer coatings covalently grafted from a wide range of medical devices.
ISSN: 2374-7951
DOI: 10.1021/acscentsci.0c00755
Rights: © 2020 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SCBE Journal Articles
SPMS Journal Articles

Citations 20

Updated on Dec 26, 2021

Citations 20

Updated on Dec 24, 2021

Page view(s)

Updated on Jan 19, 2022


Updated on Jan 19, 2022

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.