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dc.contributor.authorBharatula, Lakshmi Deepikaen_US
dc.contributor.authorMarsili, Enricoen_US
dc.contributor.authorKwan, James J.en_US
dc.identifier.citationBharatula, L. D., Marsili, E. & Kwan, J. J. (2020). Impedimetric detection of Pseudomonas aeruginosa attachment on flexible ITO-coated polyethylene terephthalate substrates. Electrochimica Acta, 332, 135390-.
dc.description.abstractBiofilm monitoring in environmental and biomedical applications remains a challenge. Currently, conventional biochemical methods do not provide a quick quantitative measure of attached biomass. Thus, there is a need for rapid in situ detection tools for routine biofilm characterization. Electrochemical impedance spectroscopy (EIS) characterizes the electroactivity of bacteria within a biofilm and has been extensively used to monitor strong electroactive biofilms. Yet, studies on weak electricigens such as Pseudomonas aeruginosa remain underrepresented. Here, conductive indium tin oxide coated polyethylene terephthalate (ITO:PET) sheets were used as flexible growth substrates instead of more conventional carbonaceous or gold materials. EIS was compared with standard optical methods for the detection of P. aeruginosa biofilms formed on ITO:PET under static growth conditions. Relaxation time analysis showed a dominant time constant at approximately 1 s and confirmed the validity of a two time constant equivalent circuit model for the biofilm impedance. The interfacial resistance calculated from the equivalent circuit analysis showed a rapid drop after bacterial attachment whereas capacitance of the biofilm was masked by the capacitance of ITO:PET. The trends for interfacial resistance and capacitance were independent to the geometry of the ITO:PET working electrode. Moreover, most robust behaviour was observed for rectangular electrodes. EIS across a broad range of potentials with and without inhibitors showed a marked difference between the interfacial resistance of viable and energy inhibited biofilms. Moreover, EIS of exopolysaccharide Δpsl mutant showed a substantial drop in current. Overall, our results indicated that EIS enabled the detection of biofilm formation across large surface areas as early as 24 h after inoculation for the weak electroactive species P. aeruginosa using a flexible polymeric substrate.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.description.sponsorshipNational Medical Research Council (NMRC)en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.relation.ispartofElectrochimica Actaen_US
dc.rights© 2019 Elsevier Ltd. All rights reserved.en_US
dc.subjectEngineering::Chemical engineeringen_US
dc.titleImpedimetric detection of Pseudomonas aeruginosa attachment on flexible ITO-coated polyethylene terephthalate substratesen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineering (SCELSE)en_US
dc.subject.keywordsElectrochemical Impedance Spectroscopyen_US
dc.description.acknowledgementThis work was financially supported by Nanyang Technological University Start-Up Grant (M4081814) and Singapore Centre for Environmental Life Sciences Engineering (SCELSE), whose research is supported by the National Research Foundation Singapore, Ministry of Education, Nanyang Technological University and National University of Singapore, under its Research Centre of Excellence Programme. This research is also supported by the Singapore Ministry of Health’s National Medical Research Council under its NMRC/OFYIRG/0034/2017.en_US
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