Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/154287
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dc.contributor.authorChong, Shi Min Sherilynen_US
dc.contributor.authorKamariah, Neelagandanen_US
dc.contributor.authorGrüber, Gerharden_US
dc.date.accessioned2021-12-16T08:25:45Z-
dc.date.available2021-12-16T08:25:45Z-
dc.date.issued2020-
dc.identifier.citationChong, S. M. S., Kamariah, N. & Grüber, G. (2020). Residues of helix ɑ2 are critical for catalytic efficiency of mycobacterial alkylhydroperoxide reductase subunit C. FEBS Letters, 594(17), 2829-2839. https://dx.doi.org/10.1002/1873-3468.13864en_US
dc.identifier.issn0014-5793en_US
dc.identifier.urihttps://hdl.handle.net/10356/154287-
dc.description.abstractThe ability of Mycobacteria to overcome oxidative stress is of paramount importance for its survival within the host. One of the key enzymes that are involved in protecting the bacterium from reactive oxygen species is the catalase-peroxidase (KatG). However, in strains resistant to the antibiotic isoniazid, KatG is rendered ineffective, which is associated with an increased expression of alkylhydroperoxide reductase subunit C (AhpC). Mycobacterial AhpC possesses a unique helical displacement when compared to its bacterial counterparts. Here, via mutagenesis studies, we demonstrate the importance of this helix for redox modulation of the catalytic activity of AhpC. Along with structural insights from crystallographic data, the impact of critical residues on the structure and flexibility of the helix and on AhpC oligomerization is described.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.language.isoenen_US
dc.relationID889en_US
dc.relation.ispartofFEBS Lettersen_US
dc.rights© 2020 Federation of European Biochemical Societies. All rights reserved.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleResidues of helix ɑ2 are critical for catalytic efficiency of mycobacterial alkylhydroperoxide reductase subunit Cen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.contributor.schoolInterdisciplinary Graduate School (IGS)en_US
dc.contributor.researchNanyang Institute of Technology in Health and Medicineen_US
dc.identifier.doi10.1002/1873-3468.13864-
dc.identifier.pmid32557576-
dc.identifier.scopus2-s2.0-85088089382-
dc.identifier.issue17en_US
dc.identifier.volume594en_US
dc.identifier.spage2829en_US
dc.identifier.epage2839en_US
dc.subject.keywordsAlkylhydroperoxide Reductaseen_US
dc.subject.keywordsMycobacteriaen_US
dc.description.acknowledgementThis study was supported by the AcademicResearch Fund (AcRF) Tier 1 ID889 Ministry of Edu-cation, Singapore, to GG, SMSC is grateful to receivean NTU Research Scholarship at Nanyang Technolog-ical University, Singapore.en_US
item.grantfulltextnone-
item.fulltextNo Fulltext-
Appears in Collections:IGS Journal Articles
SBS Journal Articles
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