Please use this identifier to cite or link to this item:
|Title:||Microfluidic-directed self-assembly of liposomes : role of interdigitation||Authors:||Lim, Shaun W. Z.
Wong, Yee Shan
|Keywords:||Engineering::Materials||Issue Date:||2020||Source:||Lim, S. W. Z., Wong, Y. S., Czarny, B. & Venkatraman, S. (2020). Microfluidic-directed self-assembly of liposomes : role of interdigitation. Journal of Colloid and Interface Science, 578, 47-57. https://dx.doi.org/10.1016/j.jcis.2020.05.114||Journal:||Journal of Colloid and Interface Science||Abstract:||Microfluidics has been used to process self-assembling liposomal systems that are commonly considered for drug delivery applications. However, it has been found that the parameters of the process are not universally suited for all lipid types. We hypothesize here that size aggregation and instability of microfluidic liposomes are a direct consequence of the presence of interdigitation in these liposomes. Interdigitation refers to the phenomenon where two opposing leaflets of a bilayer interpenetrate into one another and form a single layer. When this happens, aggregation results as the single layer is not thermodynamically stable. Such interdigitation can be induced by pressure, chemicals or by the type of lipid structure. In this study, we systematically investigate the role of lipid composition on membrane interdigitation in order to understand the dependency of lipid interdigitation on liposome formation by microfluidics. By doing so, we use nano DSC and SAXS to probe the extent of lipid interdigitation by measuring the changes in thermodynamics and membrane thickness of the lipid bilayers. Our results show that microfluidic-fabricated liposomes undergo chemical interdigitation in the presence of ethanol, in particular saturated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Strategies to prevent interdigitation is to either remove ethanol above the lipid's main transition temperature (Tm), preventing the formation of interdigitated structures and subsequent aggregated states or by the incorporation of the inhibiting additives, such as cholesterol.||URI:||https://hdl.handle.net/10356/154544||ISSN:||1095-7103||DOI:||10.1016/j.jcis.2020.05.114||Rights:||© 2020 Elsevier Inc. All rights reserved.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||MSE Journal Articles|
Updated on Dec 28, 2021
Updated on Jan 20, 2022
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.