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Title: Metabolic traits of Candidatus accumulibacter clade IIF strain SCELSE-1 using amino acids as carbon sources for enhanced biological phosphorus removal
Authors: Qiu, Guanglei
Liu, Xianghui
Saw, Nay Min Min Thaw
Law, Yingyu
Zuniga-Montanez, Rogelio
Thi, Sara Swa
Thi, Ngoc Nguyen Quynh
Nielsen, Per Halkjær
Williams, Rohan Benjamin Hugh
Wuertz, Stefan
Keywords: Engineering::Environmental engineering
Issue Date: 2020
Source: Qiu, G., Liu, X., Saw, N. M. M. T., Law, Y., Zuniga-Montanez, R., Thi, S. S., Thi, N. N. Q., Nielsen, P. H., Williams, R. B. H. & Wuertz, S. (2020). Metabolic traits of Candidatus accumulibacter clade IIF strain SCELSE-1 using amino acids as carbon sources for enhanced biological phosphorus removal. Environmental Science and Technology, 54(4), 2448-2458.
Project: 1102−IRIS−10−02
Journal: Environmental Science and Technology 
Abstract: Despite recent evidence from full-scale plants suggesting that Candidatus Accumulibacter may be capable of using amino acids, this metabolic trait has never been confirmed in a bioreactor experiment. Here we show that an enriched culture of Ca. Accumulibacter clade IIF strain SCELSE-1 could metabolize 11 of 20 α-amino acids, with aspartate, glutamate, asparagine, and glutamine resulting in the highest phosphorus removal. The anaerobic uptake of aspartate and glutamate was achieved through a glutamate/aspartate-proton symporter fully powered by the proton motive force (PMF). Under anaerobic conditions aspartate was deaminized and routed into core carbon metabolic pathways to form polyhydroxyalkanoates (PHA). The lack of genes encoding NADH dependent isocitrate dehydrogenase in the Ca. Accumulibacter genome resulted in a kinetic barrier for glutamate to be channelled to the TCA cycle. Glutamate was stored as glutamate polymer. When amino acids (aspartate or glutamate) and acetate were supplied together, Ca. Accumulibacter took up both carbon sources simultaneously, with the uptake rate of each carbon source largely preserved. Overall energy savings (up to 17%) were achieved under mixed carbon scenarios, due to the ability of Ca. Accumulibacter to rearrange its anaerobic carbon metabolism based on the reducing power, PMF and ATP balance.
ISSN: 0013-936X
DOI: 10.1021/acs.est.9b02901
Rights: © 2019 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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