Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/154951
Title: FUT6 deficiency compromises basophil function by selectively abrogating their sialyl-Lewis x expression
Authors: Puan, Kia Joo
San Luis, Boris
Nurhashikin Yusof
Kumar, Dilip
Andiappan, Anand Kumar
Lee, Wendy
Cajic, Samanta
Vuckovic, Dragana
Chan, Jing De
Döllner, Tobias
Hou, Han Wei
Jiang, Yunxuan
Tian, Chao
Rapp, Erdmann
Poidinger, Michael
Wang, De Yun
Soranzo, Nicole
Lee, Bernett
Rötzschke, Olaf
Keywords: Science::Medicine
Engineering::Mechanical engineering
Issue Date: 2021
Source: Puan, K. J., San Luis, B., Nurhashikin Yusof, Kumar, D., Andiappan, A. K., Lee, W., Cajic, S., Vuckovic, D., Chan, J. D., Döllner, T., Hou, H. W., Jiang, Y., Tian, C., Rapp, E., Poidinger, M., Wang, D. Y., Soranzo, N., Lee, B. & Rötzschke, O. (2021). FUT6 deficiency compromises basophil function by selectively abrogating their sialyl-Lewis x expression. Communications Biology, 4(1), 832-. https://dx.doi.org/10.1038/s42003-021-02295-8
Project: SIgN-06-006
SIgN-08-020
SIgN-10-029
BMRC IAF 311006
H16/99/b0/011
NMRC/1150/2008
Journal: Communications Biology
Abstract: Sialyl-Lewis x (sLex, CD15s) is a tetra-saccharide on the surface of leukocytes required for E-selectin-mediated rolling, a prerequisite for leukocytes to migrate out of the blood vessels. Here we show using flow cytometry that sLex expression on basophils and mast cell progenitors depends on fucosyltransferase 6 (FUT6). Using genetic association data analysis and qPCR, the cell type-specific defect was associated with single nucleotide polymorphisms (SNPs) in the FUT6 gene region (tagged by rs17855739 and rs778798), affecting coding sequence and/or expression level of the mRNA. Heterozygous individuals with one functional FUT6 gene harbor a mixed population of sLex+ and sLex- basophils, a phenomenon caused by random monoallelic expression (RME). Microfluidic assay demonstrated FUT6-deficient basophils rolling on E-selectin is severely impaired. FUT6 null alleles carriers exhibit elevated blood basophil counts and a reduced itch sensitivity against insect bites. FUT6-deficiency thus dampens the basophil-mediated allergic response in the periphery, evident also in lower IgE titers and reduced eosinophil counts.
URI: https://hdl.handle.net/10356/154951
ISSN: 2399-3642
DOI: 10.1038/s42003-021-02295-8
Rights: © 2021 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
MAE Journal Articles

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