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Title: Graphene quantum dot-based nanocomposites for diagnosing cancer biomarker APE1 in living cells
Authors: Zhang, Hao
Ba, Sai
Yang, Zhaoqi
Wang, Tianxiang
Lee, Jasmine Yiqin
Li, Tianhu
Shao, Fangwei
Keywords: Science::Chemistry::Biochemistry
Issue Date: 2020
Source: Zhang, H., Ba, S., Yang, Z., Wang, T., Lee, J. Y., Li, T. & Shao, F. (2020). Graphene quantum dot-based nanocomposites for diagnosing cancer biomarker APE1 in living cells. ACS Applied Materials and Interfaces, 12(12), 13634-13643.
Project: RG122/18(S)
RG117/ 17(S)
SERC A1883c0007
Journal: ACS Applied Materials and Interfaces 
Abstract: As an essential DNA repair enzyme, apurinic/apyrimidinic endonuclease 1 (APE1) is overexpressed in most human cancers and is identified as a cancer diagnostic and predictive biomarker for cancer risk assessment, diagnosis, prognosis, and prediction of treatment efficacy. Despite its importance in cancer, however, it is still a significant challenge nowadays to sense abundance variation and monitor enzymatic activity of this biomarker in living cells. Here, we report our construction of biocompatible functional nanocomposites, which are a combination of meticulously designed unimolecular DNA and fine-sized graphene quantum dots. Upon utilization of these nanocomposites as diagnostic probes, massive accumulation of fluorescence signal in living cells can be triggered by merely a small amount of cellular APE1 through repeated cycles of enzymatic catalysis. Most critically, our delicate structural designs assure that these graphene quantum dot-based nanocomposites are capable of sensing cancer biomarker APE1 in identical type of cells under different cell conditions and can be applied to multiple cancerous cells in a highly sensitive and specific manners. This work not only brings about new methods for cytology-based cancer screening but also lays down a general principle for fabricating diagnostic probes that target other endogenous biomarkers in living cells.
ISSN: 1944-8244
DOI: 10.1021/acsami.9b21385
Rights: © 2020 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SPMS Journal Articles

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