Please use this identifier to cite or link to this item:
Title: Patterning of oncogenic Ras clustering in live cells using vertically aligned nanostructure arrays
Authors: Mu, Huanwen
Zeng, Yongpeng
Zhuang, Yinyin
Gao, Weibo
Zhou, Yong
Rajalingam, Krishnaraj
Zhao, Wenting
Keywords: Engineering::Chemical engineering
Issue Date: 2022
Source: Mu, H., Zeng, Y., Zhuang, Y., Gao, W., Zhou, Y., Rajalingam, K. & Zhao, W. (2022). Patterning of oncogenic Ras clustering in live cells using vertically aligned nanostructure arrays. Nano Letters, 22(3), 1007-1016.
Project: RG145/18 and RG112/20
Journal: Nano Letters
Abstract: As a dominant oncogenic protein, Ras is well-known to segregate into clusters on the plasma membrane for activating downstream signaling. However, current technologies for direct measurements of Ras clustering are limited to sophisticated high-resolution techniques like electron microscopy and fluorescence lifetime imaging. To further promote fundamental investigations and the related drug development, we hereby introduce a nanobar-based platform which effectively guides Ras clusters into quantifiable patterns in live cells that is resolvable under conventional microscopy. Major Ras isoforms, K-Ras, H-Ras, and N-Ras were differentiated, as well as their highly prevalent oncogenic mutants G12V and G13D. Moreover, the isoform specificity and the sensitivity of a Ras inhibitor were successfully characterized on nanobars. We envision that this nanobar-based platform will serve as an effective tool to read Ras clustering on the plasma membrane, enabling a novel avenue both to decipher Ras regulations and to facilitate anti-Ras drug development.
ISSN: 1530-6984
DOI: 10.1021/acs.nanolett.1c03886
Schools: School of Chemical and Biomedical Engineering 
School of Physical and Mathematical Sciences 
Research Centres: The Photonics Institute 
Centre for Disruptive Photonic Technologies (CDPT) 
Ageing Research Institute for Society and Education
Rights: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles
SPMS Journal Articles

Files in This Item:
File Description SizeFormat 
Ras nanopillar patterning_maintext_no markup_final.pdfMaintext1.08 MBAdobe PDFThumbnail
Ras nanopillar patterning_supporting_20220119_hw.pdfSupporting Information768.01 kBAdobe PDFThumbnail

Citations 50

Updated on May 30, 2023

Web of ScienceTM
Citations 20

Updated on Jun 1, 2023

Page view(s)

Updated on Jun 6, 2023

Download(s) 50

Updated on Jun 6, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.