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Title: Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds
Authors: Koh, Kenrick
Wang, Jun Kit
Gabryelczyk, Bartosz
Tay, Dalton
Tan, Nguan Soon
Miserez, Ali
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Koh, K., Wang, J. K., Gabryelczyk, B., Tay, D., Tan, N. S. & Miserez, A. (2022). Squid suckerin-spider silk fusion protein hydrogel for stem cell-secretome delivery in chronic wounds. 2022 MRS Spring Meeting & Exhibit.
metadata.dc.contributor.conference: 2022 MRS Spring Meeting & Exhibit
Abstract: The squid sucker ring teeth, used in tandem with the suckers on the squid’s tentacles to capture prey, is comprised of mainly suckerin, a protein material. Squid suckerin is a promising biopolymer for wound dressings due to its biocompatibility, antibacterial properties, and its ability to form gels/films of varying mechanical properties. However, issues such as low recombinant solubility and the requirement of harsh photochemical means for gel/film formation hinders suckerin’s suitability for encapsulation and delivery of stem cell-secretome, which is a bioactive yet sensitive therapeutic shown to overcome persistent wound inflammation and accelerate wound healing. Via bioinspired design, we developed a novel squid suckerin-spider silk fusion protein hydrogel, where the fusion of key spider silk sequences conferred high solubility and heat-induced gelation properties that enabled encapsulation of secretome without denaturation. The protein hydrogel is capable of long-term delivery of secretome via protein-protein interaction. Additionally, due to its modular design, we incorporated cell adhesion peptides to promote biocompatibility of the hydrogel. In our results, we demonstrate the secretome-loaded hydrogel potential for clinical use via accelerated healing of excisional chronic wounds in vivo.
Schools: Interdisciplinary Graduate School (IGS) 
Rights: © 2022 Materials Research Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:IGS Conference Papers

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