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Title: Inflammation increases susceptibility of human small airway epithelial cells to pneumonic nanotoxicity
Authors: Wu, Zhuoran
Shi, Pujiang
Lim, Hong Kit
Ma, Yiyuan
Setyawati, Magdiel Inggrid
Bitounis, Dimitrios
Demokritou, Philip
Ng, Kee Woei
Tay, Chor Yong
Keywords: Engineering::Materials
Issue Date: 2020
Source: Wu, Z., Shi, P., Lim, H. K., Ma, Y., Setyawati, M. I., Bitounis, D., Demokritou, P., Ng, K. W. & Tay, C. Y. (2020). Inflammation increases susceptibility of human small airway epithelial cells to pneumonic nanotoxicity. Small, 16(21), 2000963-.
Project: NTU-HSPH18002
Journal: Small
Abstract: Exposure to inhaled anthropogenic nanomaterials (NM) with dimension <100 nm has been implicated in numerous adverse respiratory outcomes. Although studies have identified key NM physiochemical determinants of pneumonic nanotoxicity, the complex interactive and cumulative effects of NM exposure, especially in individuals with preexisting inflammatory respiratory diseases, remain unclear. Herein, the susceptibility of primary human small airway epithelial cells (SAEC) exposed to a panel of reference NM, namely, CuO, ZnO, mild steel welding fume (MSWF), and nanofractions of copier center particles (Nano-CCP), is examined in normal and tumor necrosis factor alpha (TNF-α)-induced inflamed SAEC. Compared to normal SAEC, inflamed cells display an increased susceptibility to NM-induced cytotoxicity by 15-70% due to a higher basal level of intracellular reactive oxygen species (ROS). Among the NM screened, ZnO, CuO, and Nano-CCP are observed to trigger an overcompensatory response in normal SAEC, resulting in an increased tolerance against subsequent oxidative insults. However, the inflamed SAEC fails to adapt to the NM exposure due to an impaired nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated cytoprotective response. The findings reveal that susceptibility to pulmonary nanotoxicity is highly dependent on the interplay between NM properties and inflammation of the alveolar milieu.
ISSN: 1613-6810
DOI: 10.1002/smll.202000963
Schools: School of Materials Science and Engineering 
School of Biological Sciences 
Organisations: Skin Research Institute of Singapore
Research Centres: Environmental Chemistry and Materials Centre
Nanyang Environment and Water Research Institute 
Rights: © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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