Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/155533
Title: Discovery, engineering and functional study of asparagine-specific ligases from plants
Authors: Chen, Yu
Keywords: Science::Biological sciences
Issue Date: 2021
Publisher: Nanyang Technological University
Source: Chen, Y. (2021). Discovery, engineering and functional study of asparagine-specific ligases from plants. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/155533
Abstract: Asparaginyl endopeptidases (AEPs) are cysteine proteases cutting peptide bonds after Asx (Asn/Asp). Peptide Asx-specific ligases (PALs) are AEP-like peptide ligases reversing the proteolysis of AEPs and making peptide bonds at the Asx sites. Peptide ligases have considerable potentials in biotechnology, material sciences, and precision biomanufacturing of biologics. The objective of my thesis comprises two parts. The first part focuses on the discovery of novel PALs through screening ligases from seeds and data mining search. The second part focuses on the engineering of AEP on the ligase activity determinants (LADs), which reside in the substrate-binding pockets flanking at the active pocket. Together, my thesis provides further insights to understand the occurrence and molecular determinants of peptide ligases which are useful tools for site-specific labeling proteins and targeted drug development.
URI: https://hdl.handle.net/10356/155533
DOI: 10.32657/10356/155533
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: embargo_20240229
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

Files in This Item:
File Description SizeFormat 
CHEN YU_thesis.pdf
  Until 2024-02-29
65.36 MBAdobe PDFUnder embargo until Feb 29, 2024

Page view(s)

68
Updated on Jun 25, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.