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|Title:||Splicing role of PRPF40A/B paralogs in human myeloid cells||Authors:||Tan, Cheryl Weiqi||Keywords:||Science::Biological sciences::Molecular biology||Issue Date:||2021||Publisher:||Nanyang Technological University||Source:||Tan, C. W. (2021). Splicing role of PRPF40A/B paralogs in human myeloid cells. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/155738||Abstract:||PRPF40A and PRPF40B are the human orthologs of the essential yeast splicing factor Prp40, however their splicing role is not clearly understood. The expression of PRPF40A and PRPF40B is high and low respectively in acute myeloid leukemia (AML) as compared to solid tumors. This study explored the splicing role of PRPF40A/B paralogs in AML cell maintenance, myeloid cell differentiation and immune response. Upon PRPF40A knockdown, HL-60 cells displayed increased cell death, decreased proliferation and slight differentiation phenotype with upregulation of immune activation genes. Interestingly, cell death but not proliferation was rescued with PRPF40B overexpression. Transcriptomic analysis of PRPF40A knockdown cells revealed that 80% of cassette exons were downregulated, implying its role as a splicing activator. Overall, PRPF40A and PRPF40B showed largely differing function and targets with potential implications for AML.||URI:||https://hdl.handle.net/10356/155738||DOI:||10.32657/10356/155738||Rights:||This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).||Fulltext Permission:||embargo_20240315||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Theses|
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|Cheryl PhD Thesis Final Submission.pdf|
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Updated on May 20, 2022
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