Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/156242
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dc.contributor.authorKok, Vanice Yu Qien_US
dc.date.accessioned2022-04-08T13:35:47Z-
dc.date.available2022-04-08T13:35:47Z-
dc.date.issued2022-
dc.identifier.citationKok, V. Y. Q. (2022). The self-assembly of polypeptides and their hydrolase-like activity. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/156242en_US
dc.identifier.urihttps://hdl.handle.net/10356/156242-
dc.description.abstractThis project intends to investigate the relationship between the self-assembly and hydrolase-like activity of polypeptide poly(lysine)n-b-poly(leucine)m (KmLn). Various types of KmLn polypeptide were explored using acetates such as p-nitrophenyl acetate and 4-nitrophenyl butyrate were investigated as well. The polypeptides were made inhouse before diluting them at different concentrations. UV-Vis spectroscopy was then used to monitor the production rate at which the enzymes would be statured with the substrate for a specific amount of time within the mixture. After that, the data would be transferred to a program, Origin Labs, to decipher the data and understand the enzyme-catalysed reactions using Michaelis-Menten kinetics. The results obtained shows that the production rate and enzyme affinity for each type of acetates used varies greatly. Further modification and improvement on the polypeptides were mentioned to further explore and encourage research based on this project.en_US
dc.language.isoenen_US
dc.publisherNanyang Technological Universityen_US
dc.subjectEngineering::Manufacturing::Polymers and plasticsen_US
dc.titleThe self-assembly of polypeptides and their hydrolase-like activityen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorYu Jingen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.degreeBachelor of Engineering (Materials Engineering)en_US
dc.contributor.supervisoremailyujing@ntu.edu.sgen_US
item.grantfulltextrestricted-
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Appears in Collections:MSE Student Reports (FYP/IA/PA/PI)
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