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Title: Site directed mutagenesis of alkyl hydroperoxide reductase.
Authors: Tee, Chiu Seng.
Keywords: DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2009
Abstract: This research was designed to investigate the binding site of nicotinamide adenine dinucleotide (NADH) on alkyl hydroperoxide reductase subunit F (AhpF) of Salmonella typhimurium by using site directed mutagenesis. The postulated binding site was suggested by using molecular mechanics Poisson–Boltzmann surface area (MM/PBSA) approach. AhpF enzyme requires NADH as a cofactor to function properly in peroxide reductase catalysis. The wild type AhpF was mutated in such a way that it could no longer bind to NADH as postulated by MM/PBSA approach. The hypothetical binding site would be proven correct if the activity of the mutant enzyme decreases significantly from the wild type.
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Student Reports (FYP/IA/PA/PI)

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