Please use this identifier to cite or link to this item:
|Title:||Investigating the cross-species invasion events of monkey malaria parasites into human red blood cells||Authors:||Devakumar, Aishwarya||Keywords:||Science::Biological sciences::Microbiology
Science::Biological sciences::Molecular biology
|Issue Date:||2022||Publisher:||Nanyang Technological University||Source:||Devakumar, A. (2022). Investigating the cross-species invasion events of monkey malaria parasites into human red blood cells. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/156864||Abstract:||Plasmodium knowlesi (P. knowlesi), a zoonotic malaria parasite, causes potentially severe symptoms and has shown an increasing incidence of cross-species transmissions in Southeast Asia. Despite the growing concerns of P. knowlesi infections in human red blood cells (RBCs), its invasion events and host-parasites interactions are still unclear. Thus, previous methods used to study P. falciparum invasion were implemented to investigate P. knowlesi blood-stage invasion event. Interestingly, in the presence of cyclic adenosine monophosphate (cAMP) signalling inhibitors, fluorescence plate reader and live microscopy, P. knowlesi demonstrated to activate the same Ca2+ signalling pathway in RBC as P. falciparum via cAMP signalling. G-protein and cAMP-dependent protein kinase catalytic subunit alpha (PKA) are essential members of the pathway. In P. falciparum, the species-specific protein reticulocyte-binding homolog 5 (RH5) binds to basigin to trigger RBC Ca2+ influx but attempts to identify an RH5-like protein were unsuccessful in P. knowlesi. Nevertheless, this study proves the invasion event into human RBC is conserved between species and the ligand that triggers RBC Ca2+ flux in P. knowlesi invasion is likely a membrane protein. Hence, this knowledge can facilitate the development of interventions and reveals information regarding an essential host cell signalling conservation during invasion of Plasmodium parasites.||URI:||https://hdl.handle.net/10356/156864||Fulltext Permission:||restricted||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Student Reports (FYP/IA/PA/PI)|
Updated on May 17, 2022
Updated on May 17, 2022
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.