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Title: Characterization of the strain-rate-dependent mechanical response of single cell-cell junctions
Authors: Esfahani, Amir Monemian
Rosenbohm, Jordan
Safa, Bahareh Tajvidi
Lavrik, Nickolay V.
Minnick, Grayson
Zhou, Quan
Kong, Fang
Jin, Xiaowei
Kim, Eunju
Liu, Ying
Lu, Yongfeng
Lim, Jung Yul
Wahl, James K.
Dao, Ming
Huang, Changjin
Yang, Ruiguo
Keywords: Engineering::Mechanical engineering
Issue Date: 2021
Source: Esfahani, A. M., Rosenbohm, J., Safa, B. T., Lavrik, N. V., Minnick, G., Zhou, Q., Kong, F., Jin, X., Kim, E., Liu, Y., Lu, Y., Lim, J. Y., Wahl, J. K., Dao, M., Huang, C. & Yang, R. (2021). Characterization of the strain-rate-dependent mechanical response of single cell-cell junctions. Proceedings of the National Academy of Sciences of the United States of America, 118(7), e2019347118-.
Project: M4082352.050
Journal: Proceedings of the National Academy of Sciences of the United States of America
Abstract: Cell-cell adhesions are often subjected to mechanical strains of different rates and magnitudes in normal tissue function. However, the rate-dependent mechanical behavior of individual cell-cell adhesions has not been fully characterized due to the lack of proper experimental techniques and therefore remains elusive. This is particularly true under large strain conditions, which may potentially lead to cell-cell adhesion dissociation and ultimately tissue fracture. In this study, we designed and fabricated a single-cell adhesion micro tensile tester (SCAµTT) using two-photon polymerization and performed displacement-controlled tensile tests of individual pairs of adherent epithelial cells with a mature cell-cell adhesion. Straining the cytoskeleton-cell adhesion complex system reveals a passive shear-thinning viscoelastic behavior and a rate-dependent active stress-relaxation mechanism mediated by cytoskeleton growth. Under low strain rates, stress relaxation mediated by the cytoskeleton can effectively relax junctional stress buildup and prevent adhesion bond rupture. Cadherin bond dissociation also exhibits rate-dependent strengthening, in which increased strain rate results in elevated stress levels at which cadherin bonds fail. This bond dissociation becomes a synchronized catastrophic event that leads to junction fracture at high strain rates. Even at high strain rates, a single cell-cell junction displays a remarkable tensile strength to sustain a strain as much as 200% before complete junction rupture. Collectively, the platform and the biophysical understandings in this study are expected to build a foundation for the mechanistic investigation of the adaptive viscoelasticity of the cell-cell junction.
ISSN: 0027-8424
DOI: 10.1073/pnas.2019347118
Rights: © 2021 The Author(s) (Published by National Academy of Sciences). All rights reserved. This paper was published in Proceedings of the National Academy of Sciences of the United States of America and is made available with permission of The Author(s).
Fulltext Permission: open
Fulltext Availability: With Fulltext
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