Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/157046
Title: Structural insights into viral RNA capping and plasma membrane targeting by Chikungunya virus nonstructural protein 1
Authors: Zhang, Kuo
Law, Yee-Song
Law, Michelle Cheok Yien
Tan, Yaw Bia
Wirawan, Melissa
Luo, Dahai
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Zhang, K., Law, Y., Law, M. C. Y., Tan, Y. B., Wirawan, M. & Luo, D. (2021). Structural insights into viral RNA capping and plasma membrane targeting by Chikungunya virus nonstructural protein 1. Cell Host & Microbe, 29(5), 757-764. https://dx.doi.org/10.1016/j.chom.2021.02.018
Project: MOE2016-T2- 2-097
2018-T1-002-010
NMRC/ OFIRG/0075/2018
Journal: Cell Host & Microbe
Abstract: Chikungunya virus (CHIKV) causes a debilitating arthralgic inflammatory disease in humans. The multifunctional CHIKV protein, nsP1, facilitates virus RNA replication and transcription by anchoring the viral replication complex (RC) to plasma membrane vesicles and synthesizing the viral RNA 5' cap-0. Here, we report a cryo-EM structure of CHIKV nsP1 at 2.38 Å resolution. Twelve copies of nsP1 form a crown-shaped ring structure with a 7.5-nm-wide channel for mediating communication and exchange between the viral RC and the host cell. The catalytic site for viral RNA capping is located in a tunnel that is shaped by neighboring nsP1 molecules. Two membrane-association loops target nsP1 to the inner leaflet of the plasma membrane via palmitoylation and hydrophobic and electrostatic interactions. Our study provides the structural basis of viral RNA capping and RC assembly mediated by nsP1 and guides the development of antivirals targeting these essential steps of virus infection.
URI: https://hdl.handle.net/10356/157046
ISSN: 1931-3128
DOI: 10.1016/j.chom.2021.02.018
Rights: © 2021 Elsevier Inc. All rights reserved. This paper was published in Cell Host & Microbe and is made available with permission of Elsevier Inc.
Fulltext Permission: embargo_20220519
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles

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