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Title: Accuracy of peak-power compensation in fiber-guided and free-space acoustic-resolution photoacoustic microscopy
Authors: Asadollahi, Amir
Latifi, Hamid
Zeynali, Shahriar
Pramanik, Manojit
Qazvini, Hamed
Keywords: Engineering::Bioengineering
Issue Date: 2022
Source: Asadollahi, A., Latifi, H., Zeynali, S., Pramanik, M. & Qazvini, H. (2022). Accuracy of peak-power compensation in fiber-guided and free-space acoustic-resolution photoacoustic microscopy. Biomedical Optics Express, 13(3), 1774-1783.
Journal: Biomedical Optics Express
Abstract: Acoustic resolution photoacoustic microscopy (AR-PAM) has gained much attention in the past two decades due to its high contrast, scalable resolution, and relatively higher imaging depth. Multimode optical fibers (MMF) are extensively used to transfer light to AR-PAM imaging scan-head from the laser source. Typically, peak-power-compensation (PPC) is used to reduce the effect of pulse-to-pulse peak-power variation in generated photoacoustic (PA) signals. In MMF, the output intensity profile fluctuates due to the coherent nature of light and mode exchange caused by variations in the bending of the fibers during scanning. Therefore, using a photodiode (PD) to capture a portion of the total power of pulses as a measure of illuminated light on the sample may not be appropriate for accurate PPC. In this study, we have investigated the accuracy of PPC in fiber-guided and free-space AR-PAM systems. Experiments were conducted in the transparent and highly scattering medium. Based on obtained results for the MMF-based system, to apply PPC to the generated PA signals, tightly focused light confocal with the acoustic focus in a transparent medium must be used. In the clear medium and highly focused illumination, enhancement of about 45% was obtained in the homogeneity of an optically homogeneous sample image. In addition, it is shown that, as an alternative, free-space propagation of the laser pulses results in more accurate PPC in both transparent and highly scattering mediums. In free-space light transmission, enhancement of 25-75% was obtained in the homogeneity of the optically homogeneous sample image.
ISSN: 2156-7085
DOI: 10.1364/BOE.453475
Rights: © 2022 Optica Publishing Group under the terms of the Open Access Publishing Agreement. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for noncommercial purposes and appropriate attribution is maintained. All other rights are reserved.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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