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Title: Printer center nanoparticles alter the DNA repair capacity of human bronchial airway epithelial cells
Authors: Bitounis, Dimitrios
Huang, Qiansheng
Toprani, Sneh M.
Setyawati, Magdiel Inggrid
Oliveira, Nathalia
Wu, Zhuoran
Tay, Chor Yong
Ng, Kee Woei
Nagel, Zachary D.
Demokritou, Philip
Keywords: Engineering::Materials
Issue Date: 2022
Source: Bitounis, D., Huang, Q., Toprani, S. M., Setyawati, M. I., Oliveira, N., Wu, Z., Tay, C. Y., Ng, K. W., Nagel, Z. D. & Demokritou, P. (2022). Printer center nanoparticles alter the DNA repair capacity of human bronchial airway epithelial cells. NanoImpact, 25, 100379-.
Project: NTU-HSPH 18002 
Journal: NanoImpact 
Abstract: Nano-enabled, toner-based printing equipment emit nanoparticles during operation. The bioactivity of these nanoparticles as documented in a plethora of published toxicological studies raises concerns about their potential health effects. These include pro-inflammatory effects that can lead to adverse epigenetic alterations and cardiovascular disorders in rats. At the same time, their potential to alter DNA repair pathways at realistic doses remains unclear. In this study, size-fractionated, airborne particles from a printer center in Singapore were sampled and characterized. The PM0.1 size fraction (particles with an aerodynamic diameter less than 100 nm) of printer center particles (PCP) were then administered to human lung adenocarcinoma (Calu-3) or lymphoblastoid (TK6) cells. We evaluated plasma membrane integrity, mitochondrial activity, and intracellular reactive oxygen species (ROS) generation. Moreover, we quantified DNA damage and alterations in the cells' capacity to repair 6 distinct types of DNA lesions. Results show that PCP altered the ability of Calu-3 cells to repair 8oxoG:C lesions and perform nucleotide excision repair, in the absence of acute cytotoxicity or DNA damage. Alterations in DNA repair capacity have been correlated with the risk of various diseases, including cancer, therefore further genotoxicity studies are needed to assess the potential risks of PCP exposure, at both occupational settings and at the end-consumer level.
ISSN: 2452-0748
DOI: 10.1016/j.impact.2022.100379
Schools: School of Materials Science and Engineering 
School of Biological Sciences 
Research Centres: Nanyang Environment and Water Research Institute 
Environmental Chemistry and Materials Centre
Rights: © 2022 Elsevier B.V. All rights reserved. This paper was published in NanoImpact and is made available with permission of Elsevier B.V.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles
NEWRI Journal Articles
SBS Journal Articles

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