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Title: Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold
Authors: Devi, Gitali
Winnerdy, Fernaldo Richtia
Ang, Jason Cheng Yu
Lim, Kah Wai
Phan, Anh Tuân
Keywords: Science::Chemistry
Science::Biological sciences
Issue Date: 2022
Source: Devi, G., Winnerdy, F. R., Ang, J. C. Y., Lim, K. W. & Phan, A. T. (2022). Four-layered intramolecular parallel G-quadruplex with non-nucleotide loops: an ultra-stable self-folded DNA nano-scaffold. ACS Nano, 16, 533-540.
Project: NRF-NRFI2017-09
Journal: ACS Nano 
Abstract: A four-stranded scaffold of nucleic acids termed G-quadruplex (G4) has found growing applications in nano- and biotechnology. Propeller loops are a hallmark of the most stable intramolecular parallel-stranded G4s. To date, propeller loops have been observed to span only a maximum of three G-tetrad layers. Going beyond that would allow creation of more stable scaffolds useful for building robust nanodevices. Here we investigate the formation of propeller loops spanning more than three layers. We showed that native nucleotide sequences are incompatible to-wards this goal, and we identified synthetic non-nucleotide linkers that form propeller loop across four layers. With the established linkers, we constructed a four-layered intramolecular parallel-stranded G4, which exhibited ultra-high thermal stability. Control on loop design would augment the toolbox towards engineering of G4-based nano-scaffolds for diverse applications.
ISSN: 1936-0851
DOI: 10.1021/acsnano.1c07630
Schools: School of Physical and Mathematical Sciences 
Research Centres: NTU Institute of Structural Biology 
Rights: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SPMS Journal Articles

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