Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/159178
Title: The study on the effects of hydrogen peroxide and antibiotic-antimycotic disinfectants on the decellularization of porcine oesophagus
Authors: Yah, Jermaine Rui Ting
Keywords: Engineering::Mechanical engineering
Issue Date: 2022
Publisher: Nanyang Technological University
Source: Yah, J. R. T. (2022). The study on the effects of hydrogen peroxide and antibiotic-antimycotic disinfectants on the decellularization of porcine oesophagus. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/159178
Abstract: Decellularized pig scaffolds have shown promise in human organ transplant, which can help solve the global organ transplant shortage. The aim of decellularization is to maintain the extracellular matrix, remove donor cells entirely and retain the mechanical structure. In this project, 20mg/ml of sodium deoxycholate (2% SDC) is used in conjunction with 2mg/ml of sodium dodecyl sulfate (0.2% SDS) as both solutions are known to be effective in the decellularization process. The efficacy of 3% hydrogen peroxide was also tested as an alternative storage method to phosphate-buffered saline + 1% antibiotic-antimycotic. The porcine oesophagi are immersed in 200ml of sodium deoxycholate/ sodium dodecyl sulfate over different durations after which DNA quantification and histological results are compared to test the effectiveness of the decellularization process. The results showed that 3% hydrogen peroxide (H2O2) was effective in preventing microbial contamination and was also able to remove some DNA from the oesophagi while retaining the structure of the ECM. Hence H2O2 can be considered as a possible storage alternative.
URI: https://hdl.handle.net/10356/159178
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:MAE Student Reports (FYP/IA/PA/PI)

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