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Title: Renal-clearable molecular probe for near-infrared fluorescence imaging and urinalysis of SARS-CoV-2
Authors: Liew, Si Si
Zeng, Ziling
Cheng, Penghui
He, Shasha
Zhang, Chi
Pu, Kanyi
Keywords: Engineering::Chemical engineering
Issue Date: 2021
Source: Liew, S. S., Zeng, Z., Cheng, P., He, S., Zhang, C. & Pu, K. (2021). Renal-clearable molecular probe for near-infrared fluorescence imaging and urinalysis of SARS-CoV-2. Journal of the American Chemical Society, 143(45), 18827-18831.
Project: 2019-T1-002-045
RT05/ 20
SERC A18A8b0059
Journal: Journal of the American Chemical Society
Abstract: Despite the importance of rapid and accurate detection of SARS-CoV-2 in controlling the COVID-19 pandemic, current diagnostic methods are static and unable to distinguish between viable/nonviable virus or directly reflect viral replication activity. Real-time imaging of protease activity specific to SARS-CoV-2 can overcome these issues but remains lacking. Herein, we report a near-infrared fluorescence (NIRF) activatable molecular probe (SARS-CyCD) for detection of SARS-CoV-2 protease in living mice. The probe comprises a hemicyanine fluorophore caged with a protease peptide substrate and a cyclodextrin unit, which function as an NIRF signaling moiety and a renal-clearable enabler, respectively. The peptide substrate of SARS-CyCD can be specifically cleaved by SARS-CoV-2 main protease (Mpro), resulting in NIRF signal activation and liberation of the renal-clearable fluorescent fragment (CyCD). Such a design not only allows sensitive detection of Mpro in the lungs of living mice after intratracheal administration but also permits optical urinalysis of SARS-CoV-2 infection. Thus, this study presents an in vivo sensor that holds potential in preclinical high-throughput drug screening and clinical diagnostics for respiratory viral infections.
ISSN: 0002-7863
DOI: 10.1021/jacs.1c08017
Rights: © 2021 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles
SCBE Journal Articles
SPMS Journal Articles

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