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Title: Roseobacters in a sea of Poly- and Paraphyly: whole genome-based taxonomy of the family Rhodobacteraceae and the proposal for the split of the "roseobacter clade" into a novel family, Roseobacteraceae fam. nov
Authors: Liang, Kevin Y. H.
Orata, Fabini D.
Boucher, Yann F.
Case, Rebecca J.
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Liang, K. Y. H., Orata, F. D., Boucher, Y. F. & Case, R. J. (2021). Roseobacters in a sea of Poly- and Paraphyly: whole genome-based taxonomy of the family Rhodobacteraceae and the proposal for the split of the "roseobacter clade" into a novel family, Roseobacteraceae fam. nov. Frontiers in Microbiology, 12, 683109-.
Journal: Frontiers in Microbiology 
Abstract: The family Rhodobacteraceae consists of alphaproteobacteria that are metabolically, phenotypically, and ecologically diverse. It includes the roseobacter clade, an informal designation, representing one of the most abundant groups of marine bacteria. The rapid pace of discovery of novel roseobacters in the last three decades meant that the best practice for taxonomic classification, a polyphasic approach utilizing phenotypic, genotypic, and phylogenetic characteristics, was not always followed. Early efforts for classification relied heavily on 16S rRNA gene sequence similarity and resulted in numerous taxonomic inconsistencies, with several poly- and paraphyletic genera within this family. Next-generation sequencing technologies have allowed whole-genome sequences to be obtained for most type strains, making a revision of their taxonomy possible. In this study, we performed whole-genome phylogenetic and genotypic analyses combined with a meta-analysis of phenotypic data to review taxonomic classifications of 331 type strains (under 119 genera) within the Rhodobacteraceae family. Representatives of the roseobacter clade not only have different environmental adaptions from other Rhodobacteraceae isolates but were also found to be distinct based on genomic, phylogenetic, and in silico-predicted phenotypic data. As such, we propose to move this group of bacteria into a new family, Roseobacteraceae fam. nov. In total, reclassifications resulted to 327 species and 128 genera, suggesting that misidentification is more problematic at the genus than species level. By resolving taxonomic inconsistencies of type strains within this family, we have established a set of coherent criteria based on whole-genome-based analyses that will help guide future taxonomic efforts and prevent the propagation of errors.
ISSN: 1664-302X
DOI: 10.3389/fmicb.2021.683109
Rights: © 2021 Liang, Orata, Boucher and Case. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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