Please use this identifier to cite or link to this item:
Title: Circulating microRNA breast cancer biomarker detection in patient sera with surface plasmon resonance imaging biosensor
Authors: Wong, Chi Lok
Loke, Sau Yeen
Lim, Hann Qian
Balasundaram, Ghayathri
Chan, Patrick
Chong, Bee Kiang
Tan, Ern Yu
Lee, Ann Siew Gek
Olivo, Malini
Keywords: Science::Medicine
Issue Date: 2021
Source: Wong, C. L., Loke, S. Y., Lim, H. Q., Balasundaram, G., Chan, P., Chong, B. K., Tan, E. Y., Lee, A. S. G. & Olivo, M. (2021). Circulating microRNA breast cancer biomarker detection in patient sera with surface plasmon resonance imaging biosensor. Journal of Biophotonics, 14(11), e202100153-.
Project: ETPL/14-R15GAP-0027
Journal: Journal of Biophotonics
Abstract: In this article, we report for the first time, the detection of circulating miRNA as a breast cancer biomarker in patient sera using surface plasmon resonance imaging biosensor. The advantage of this approach lies in the rapid, label-free and sensitive detection. The sensor excites plasmonic resonance on the gold sensor surface and specific DNA-miRNA molecular bindings elucidate responses in the plasmonic resonance image. Experiments of detecting synthetic miRNA molecules (miR-1249) were performed and the sensor resolution was found to be 63.5 nM. The sensor was further applied to screen 17 patient serum samples from National Cancer Centre Singapore and Tan Tock Seng Hospital. Sensor intensity response was found to differ by 20% between malignant and benign cases and thus forms, a potential and an important metric in distinguishing benignity and malignancy.
ISSN: 1864-063X
DOI: 10.1002/jbio.202100153
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: Institute of Molecular and Cell Biology, A*STAR
Tan Tock Seng Hospital
Rights: © 2021 Wiley-VCH GmbH. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles

Citations 50

Updated on Sep 22, 2023

Web of ScienceTM
Citations 50

Updated on Sep 24, 2023

Page view(s)

Updated on Sep 27, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.