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Title: Trapping and detection of single viruses in an optofluidic chip
Authors: Shi, Yuzhi
Nguyen, Kim Truc
Chin, Lip Ket
Li, Zhenyu
Xiao, Limin
Cai, Hong
Yu, Ruozhen
Huang, Wei
Feng, Shilun
Yap, Peng Huat
Liu, Jingquan
Zhang, Yi
Liu, Ai Qun
Keywords: Engineering::Electrical and electronic engineering
Issue Date: 2021
Source: Shi, Y., Nguyen, K. T., Chin, L. K., Li, Z., Xiao, L., Cai, H., Yu, R., Huang, W., Feng, S., Yap, P. H., Liu, J., Zhang, Y. & Liu, A. Q. (2021). Trapping and detection of single viruses in an optofluidic chip. ACS Sensors, 6(9), 3445-3450.
Project: NRFCRP13-2014-01
Journal: ACS Sensors
Abstract: Accurate single virus detection is critical for disease diagnosis and early prevention, especially in view of current pandemics. Numerous detection methods have been proposed with the single virus sensitivity, including the optical approaches and immunoassays. However, few of them hitherto have the capability of both trapping and detection of single viruses in the microchannel. Here, we report an optofluidic potential well array to trap nanoparticles stably in the flow stream. The nanoparticle is bound with single viruses and fluorescence quantum dots through an immunolabeling protocol. Single viruses can be swiftly captured in the microchannel by optical forces and imaged by a camera. The number of viruses in solution and on each particle can be quantified via image processing. Our method can trap and detect single viruses in the 1 mL serum or water in 2 h, paving an avenue for the advanced, fast, and accurate clinical diagnosis, as well as the study of virus infectivity, mutation, drug inhibition, etc.
ISSN: 2379-3694
DOI: 10.1021/acssensors.1c01350
Rights: © 2021 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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