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dc.contributor.authorChandra, Franciscusen_US
dc.contributor.authorLee, Wei Linen_US
dc.contributor.authorArmas, Federicaen_US
dc.contributor.authorLeifels, Matsen_US
dc.contributor.authorGu, Xiaoqiongen_US
dc.contributor.authorChen, Hongjieen_US
dc.contributor.authorWuertz, Stefanen_US
dc.contributor.authorAlm, Eric J.en_US
dc.contributor.authorThompson, Janelleen_US
dc.identifier.citationChandra, F., Lee, W. L., Armas, F., Leifels, M., Gu, X., Chen, H., Wuertz, S., Alm, E. J. & Thompson, J. (2021). Persistence of dengue (serotypes 2 and 3), Zika, yellow fever, and murine hepatitis virus RNA in untreated wastewater. Environmental Science and Technology Letters, 8(9), 785-791.
dc.description.abstractArboviruses are viral pathogens transmitted by blood-borne vectors that impose a great social and economic burden globally. Most clinical surveillance of arbovirus outbreaks underestimates the true prevalence as a large proportion of cases exhibit no or only mild clinical symptoms (i.e., are subclinical). Reports of urinary shedding of several arboviruses such as Dengue (DENV), yellow fever (YFV), and Zika (ZIKV) viruses suggest the possibility of utilizing wastewater surveillance to assess the prevalence of arboviral outbreaks. To determine the feasibility of wastewater surveillance, we investigated the decay of representative arboviruses (including DENV-2, DENV-3, YFV, and ZIKV) along with murine hepatitis virus (MHV) as a surrogate for human coronavirus within a wastewater matrix at 6, 25, or 37 °C using RT-qPCR. DENV-2, DENV-3, YFV, ZIKV, and MHV experienced a one log10reduction within 3.95-6.21 days at 25 °C and within 2.60-5.12 days at 37 °C, while incubation at 6 °C did not indicate substantial decay within 21 days. Our work suggests that these arboviruses or their RNA could persist sufficiently in wastewater over a range of temperatures, supporting the potential for wastewater-based surveillance of arboviral outbreaks.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.description.sponsorshipSingapore-MIT Alliance for Research and Technology (SMART)en_US
dc.relation.ispartofEnvironmental Science and Technology Lettersen_US
dc.rights© 2021 The Authors. Published by American Chemical Society. All rights reserved.en_US
dc.subjectEngineering::Environmental engineeringen_US
dc.titlePersistence of dengue (serotypes 2 and 3), Zika, yellow fever, and murine hepatitis virus RNA in untreated wastewateren_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolAsian School of the Environmenten_US
dc.contributor.organizationCampus for Research Excellence and Technological Enterprise (CREATE)en_US
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineering (SCELSE)en_US
dc.description.acknowledgementThis research was supported by the National Research Foundation, Prime Minister’s Office, Singapore, under its Campus for Research Excellence and Technological Enterprise (CREATE) program, through Intra-CREATE Thematic Grant (Cities) Grant NRF2019-THE001-0003 to J.T. and E.J.A., the Singapore-MIT Alliance for Research and Technology (SMART) Antimicrobial Resistance Interdisciplinary Research Group (AMR IRG), and funding from the Singapore Ministry of Education and National Research Foundation through an RCE award to the Singapore Centre for Environmental Life Sciences Engineering (SCELSE).en_US
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