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Title: Persistence of dengue (serotypes 2 and 3), Zika, yellow fever, and murine hepatitis virus RNA in untreated wastewater
Authors: Chandra, Franciscus
Lee, Wei Lin
Armas, Federica
Leifels, Mats
Gu, Xiaoqiong
Chen, Hongjie
Wuertz, Stefan
Alm, Eric J.
Thompson, Janelle
Keywords: Engineering::Environmental engineering
Issue Date: 2021
Source: Chandra, F., Lee, W. L., Armas, F., Leifels, M., Gu, X., Chen, H., Wuertz, S., Alm, E. J. & Thompson, J. (2021). Persistence of dengue (serotypes 2 and 3), Zika, yellow fever, and murine hepatitis virus RNA in untreated wastewater. Environmental Science and Technology Letters, 8(9), 785-791.
Project: NRF2019-THE001-0003 
Journal: Environmental Science and Technology Letters 
Abstract: Arboviruses are viral pathogens transmitted by blood-borne vectors that impose a great social and economic burden globally. Most clinical surveillance of arbovirus outbreaks underestimates the true prevalence as a large proportion of cases exhibit no or only mild clinical symptoms (i.e., are subclinical). Reports of urinary shedding of several arboviruses such as Dengue (DENV), yellow fever (YFV), and Zika (ZIKV) viruses suggest the possibility of utilizing wastewater surveillance to assess the prevalence of arboviral outbreaks. To determine the feasibility of wastewater surveillance, we investigated the decay of representative arboviruses (including DENV-2, DENV-3, YFV, and ZIKV) along with murine hepatitis virus (MHV) as a surrogate for human coronavirus within a wastewater matrix at 6, 25, or 37 °C using RT-qPCR. DENV-2, DENV-3, YFV, ZIKV, and MHV experienced a one log10reduction within 3.95-6.21 days at 25 °C and within 2.60-5.12 days at 37 °C, while incubation at 6 °C did not indicate substantial decay within 21 days. Our work suggests that these arboviruses or their RNA could persist sufficiently in wastewater over a range of temperatures, supporting the potential for wastewater-based surveillance of arboviral outbreaks.
ISSN: 2328-8930
DOI: 10.1021/acs.estlett.1c00517
Schools: School of Chemical and Biomedical Engineering 
Asian School of the Environment 
Organisations: Campus for Research Excellence and Technological Enterprise (CREATE)
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) 
Rights: © 2021 The Authors. Published by American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:ASE Journal Articles
CEE Journal Articles
SCELSE Journal Articles

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