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Title: Self-assembly of dendritic DNA into a hydrogel: application in three-dimensional cell culture
Authors: Wu, Jingyuan
Liyarita, Bella Rosa
Zhu, Haishuang
Liu, Ming
Hu, Xiao
Shao, Fangwei
Keywords: Engineering::Materials
Issue Date: 2021
Source: Wu, J., Liyarita, B. R., Zhu, H., Liu, M., Hu, X. & Shao, F. (2021). Self-assembly of dendritic DNA into a hydrogel: application in three-dimensional cell culture. ACS Applied Materials and Interfaces, 13(42), 49705-49712.
Journal: ACS Applied Materials and Interfaces 
Abstract: With inherent biocompatibility, biodegradability, and unique programmability, hydrogels with a DNA framework show great potential in three-dimensional (3D) cell culture. Here, a DNA hydrogel was assembled by a dendritic DNA with four branches. The hydrogel showed tunable mechanical strength and reversible thixotropy even under a nanomolar DNA concentration. The cell culture medium can be converted into the hydrogel isothermally at physiological temperature. This DNA hydrogel allows both cancer and somatic cells to be seeded in situ and to achieve high proliferation and viability. The bis-entity of dendritic branches enabled the specific loading of bioactive clues to regulate cell behaviors. Thus, the dendritic DNA-assembled hydrogel could serve as a highly biocompatible, readily functionalizing, and easy-casting gel platform for 3D cell culture.
ISSN: 1944-8244
DOI: 10.1021/acsami.1c14445
Schools: School of Physical and Mathematical Sciences 
School of Materials Science and Engineering 
Research Centres: Nanyang Environment and Water Research Institute 
Environment Chemistry and Materials Centre
Temasek Laboratories @ NTU 
Rights: © 2021 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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