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Title: In situ formation of 3D conductive and cell-laden graphene hydrogel for electrically regulating cellular behavior
Authors: Chen, Xuelong
Ranjan, Vivek Damodar
Liu, Sijun
Liang, Yen Nan
Lim, Jacob Song Kiat
Chen, Hui
Hu, Xiao
Zhang, Yilei
Keywords: Engineering::Materials
Issue Date: 2021
Source: Chen, X., Ranjan, V. D., Liu, S., Liang, Y. N., Lim, J. S. K., Chen, H., Hu, X. & Zhang, Y. (2021). In situ formation of 3D conductive and cell-laden graphene hydrogel for electrically regulating cellular behavior. Macromolecular Bioscience, 21(4), 2000374-.
Journal: Macromolecular Bioscience 
Abstract: Electroconductive and injectable hydrogels are attracting increasing attention owing to the needs of electrically induced regulation of cell behavior, tissue engineering of electroactive tissues, and achieving minimum invasiveness during tissue repair. In this study, a novel in situ formed 3D conductive and cell-laden hydrogel is developed, which can be broadly used in bioprinting, tissue engineering, neuroengineering etc. An instantaneous, uniform spatial distribution and encapsulation of cells can be achieved as a result of hydrogen bonding induced hydrogel formation. Particularly, the cell-laden hydrogel can be easily obtained by simply mixing and shaking the polydopamine (PDA) functionalized rGO (rGO-PDA) with polyvinyl alcohol (PVA) solution containing cells. Graphene oxide is reduced and functionalized by dopamine to restore the electrical conductivity, while simultaneously enhancing both hydrophilicity and biocompatibility of reduced graphene oxide. In vitro culture of PC12 cells within the cell-laden hydrogel demonstrates its biocompatibility, noncytotoxicity as well as the ability to support long-term cell growth and proliferation. Enhanced neuronal differentiation is also observed, both with and without electrical stimulation. Overall, this 3D conductive, cell-laden hydrogel holds great promise as potential platform for tissue engineering of electroactive tissues.
ISSN: 1616-5187
DOI: 10.1002/mabi.202000374
Schools: School of Materials Science and Engineering 
Interdisciplinary Graduate School (IGS) 
Research Centres: NTU Institute for Health Technologies 
Nanyang Environment and Water Research Institute 
Temasek Laboratories @ NTU 
Rights: © 2021 Wiley-VCH GmbH. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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