Please use this identifier to cite or link to this item:
Title: Recent advances in investigating functional dynamics of chromatin
Authors: Shi, Xiangyan
Zhai, Ziwei
Chen, Yinglu
Li, Jindi
Nordenskiöld, Lars
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Shi, X., Zhai, Z., Chen, Y., Li, J. & Nordenskiöld, L. (2022). Recent advances in investigating functional dynamics of chromatin. Frontiers in Genetics, 13, 870640-.
Project: MOE2018- T2-1-112
Journal: Frontiers in Genetics
Abstract: Dynamics spanning the picosecond-minute time domain and the atomic-subcellular spatial window have been observed for chromatin in vitro and in vivo. The condensed organization of chromatin in eukaryotic cells prevents regulatory factors from accessing genomic DNA, which requires dynamic stabilization and destabilization of structure to initiate downstream DNA activities. Those processes are achieved through altering conformational and dynamic properties of nucleosomes and nucleosome-protein complexes, of which delineating the atomistic pictures is essential to understand the mechanisms of chromatin regulation. In this review, we summarize recent progress in determining chromatin dynamics and their modulations by a number of factors including post-translational modifications (PTMs), incorporation of histone variants, and binding of effector proteins. We focus on experimental observations obtained using high-resolution techniques, primarily including nuclear magnetic resonance (NMR) spectroscopy, Förster (or fluorescence) resonance energy transfer (FRET) microscopy, and molecular dynamics (MD) simulations, and discuss the elucidated dynamics in the context of functional response and relevance.
ISSN: 1664-8021
DOI: 10.3389/fgene.2022.870640
Schools: School of Biological Sciences 
Rights: © 2022 Shi, Zhai, Chen, Li and Nordenskiöld. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

Files in This Item:
File Description SizeFormat 
fgene-13-870640.pdf962.3 kBAdobe PDFThumbnail

Citations 50

Updated on May 31, 2023

Web of ScienceTM
Citations 50

Updated on Jun 1, 2023

Page view(s)

Updated on Jun 2, 2023


Updated on Jun 2, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.