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Title: Feeding state greatly modulates the effect of xenobiotics on gut microbiome metabolism: a case study of tetracycline
Authors: Keerthisinghe, Tharushi Prabha
Yang, Qin
Chow, Agnes
Fang, Mingliang
Keywords: Science::Medicine
Issue Date: 2021
Source: Keerthisinghe, T. P., Yang, Q., Chow, A. & Fang, M. (2021). Feeding state greatly modulates the effect of xenobiotics on gut microbiome metabolism: a case study of tetracycline. Journal of Hazardous Materials, 413, 125441-.
Project: 04MNP000567C120EOM01
Journal: Journal of Hazardous Materials 
Abstract: The human gut microbiome is crucial in modulating host health mostly through bacterial metabolites. Chemical exposure is typical external stress which alters its composition and functionality. To date, very few studies have investigated the effect of feeding state on chemical-induced gut microbial metabolic dysregulations. Here, we set up an in vitro human gut microbiome and incorporated a metabolomics approach to investigate the effect of tetracycline (TET) at multiple doses (i.e., 10, 1, and 0.01 mg/L) on gut microbiome under the fed and fasted states. Overall, the metabolome was highly responsive at the fed state with 62 metabolites dysregulated while only 14 were altered at the fasted state under 10 mg/L (clinical TET dose). As expected, nutrient consumption was significantly inhibited under clinical TET dose at the fed state accumulating nutrients such as glutamate and leucine. Interestingly, at the fed state, TET could increase the synthesis of indole and phenyl derivatives including indole-3-aldehyde and hydrocinnamate, while inhibiting indoxyl, tryptamine, and vitamin B production, all of which have host health implications. Furthermore, metabolites like indoxyl and xanthurenic acid were still responsive at 0.01 mg/L (dietary TET dose). Collectively, results demonstrated that the feeding state greatly modulates the chemical-induced gut microbial metabolic alterations.
ISSN: 0304-3894
DOI: 10.1016/j.jhazmat.2021.125441
Schools: School of Civil and Environmental Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Research Centres: Nanyang Environment and Water Research Institute 
Singapore Phenome Centre 
Rights: © 2021 Elsevier B.V. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:CEE Journal Articles
LKCMedicine Journal Articles
NEWRI Journal Articles

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