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Title: Oriented and sustained protein expression on biomimicking electrospun fibers for evaluating functionality of cells
Authors: Lin, Junquan
Chooi, Wai Hon
Ong, William
Zhang, Na
Bechler, Marie E.
ffrench-Constant, Charles
Chew, Sing Yian
Keywords: Engineering::Bioengineering
Issue Date: 2021
Source: Lin, J., Chooi, W. H., Ong, W., Zhang, N., Bechler, M. E., ffrench-Constant, C. & Chew, S. Y. (2021). Oriented and sustained protein expression on biomimicking electrospun fibers for evaluating functionality of cells. Materials Science and Engineering: C, 118, 111407-.
Project: A*STAR/BMRC/1610500024 
Journal: Materials Science and Engineering: C 
Abstract: A proper protein orientation is often required in order to achieve specific protein-receptor interaction to elicit a desired biological response. Here, we present a Protein A-based biomimicking platform that is capable of efficiently orienting proteins for evaluating cellular behaviour. By absorbing Protein A onto aligned bio-mimicking polycaprolactone (PCL) fibers, we demonstrate that protein binding could be retained on these fibers for at least 7 days under physiologically relevant conditions. We further show that Protein A served as a molecular orientor to arrange the recombinant proteins in similar orientations. Such protein-orienting scaffolds were further verified to be biologically functional by using sensitive primary rat cortical neurons (CNs) and oligodendrocyte progenitor cells (OPCs), as model neural cells for a stringent proof of concept. Specifically, CNs that were seeded on fibers coated with Protein A and a known enhancer of neurite growth (L1 Cell Adhesion Molecular L1CAM) displayed the longest total neurite length (462.77 ± 100.79 μm, p < 0.001) as compared to the controls. Besides that, OPCs seeded on fibers coated with Protein A and Neuregulin-1 Type III (Nrg1 type III) (myelin enhancer) produced the longest myelin ensheathment length (19.8 ± 11.69 μm). These results demonstrate the efficacy of this platform for protein screening applications.
ISSN: 0928-4931
DOI: 10.1016/j.msec.2020.111407
Schools: School of Chemical and Biomedical Engineering 
Interdisciplinary Graduate School (IGS) 
Lee Kong Chian School of Medicine (LKCMedicine) 
Research Centres: NTU Institute for Health Technologies 
Rights: © 2020 Elsevier B.V. All rights reserved. This paper was published in Materials Science and Engineering: C and is made available with permission of Elsevier B.V.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:IGS Journal Articles
LKCMedicine Journal Articles
SCBE Journal Articles

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