Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/160518
Title: Zyxin is involved in fibroblast rigidity sensing and durotaxis
Authors: Yip, Ai Kia
Zhang, Songjing
Chong, Lor Huai
Cheruba, Elsie
Woon, Jessie Yong Xing
Chua, Theng Xuan
Goh, Corinna Jie Hui
Yang, Haibo
Tay, Chor Yong
Koh, Cheng-Gee
Chiam, Keng-Hwee
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Yip, A. K., Zhang, S., Chong, L. H., Cheruba, E., Woon, J. Y. X., Chua, T. X., Goh, C. J. H., Yang, H., Tay, C. Y., Koh, C. & Chiam, K. (2021). Zyxin is involved in fibroblast rigidity sensing and durotaxis. Frontiers in Cell and Developmental Biology, 9, 735298-. https://dx.doi.org/10.3389/fcell.2021.735298
Project: 2018- T2-1-058 
RG106/20 
Journal: Frontiers in Cell and Developmental Biology 
Abstract: Focal adhesions (FAs) are specialized structures that enable cells to sense their extracellular matrix rigidity and transmit these signals to the interior of the cells, bringing about actin cytoskeleton reorganization, FA maturation, and cell migration. It is known that cells migrate towards regions of higher substrate rigidity, a phenomenon known as durotaxis. However, the underlying molecular mechanism of durotaxis and how different proteins in the FA are involved remain unclear. Zyxin is a component of the FA that has been implicated in connecting the actin cytoskeleton to the FA. We have found that knocking down zyxin impaired NIH3T3 fibroblast's ability to sense and respond to changes in extracellular matrix in terms of their FA sizes, cell traction stress magnitudes and F-actin organization. Cell migration speed of zyxin knockdown fibroblasts was also independent of the underlying substrate rigidity, unlike wild type fibroblasts which migrated fastest at an intermediate substrate rigidity of 14 kPa. Wild type fibroblasts exhibited durotaxis by migrating toward regions of increasing substrate rigidity on polyacrylamide gels with substrate rigidity gradient, while zyxin knockdown fibroblasts did not exhibit durotaxis. Therefore, we propose zyxin as an essential protein that is required for rigidity sensing and durotaxis through modulating FA sizes, cell traction stress and F-actin organization.
URI: https://hdl.handle.net/10356/160518
ISSN: 2296-634X
DOI: 10.3389/fcell.2021.735298
Rights: © 2021 Yip, Zhang, Chong, Cheruba, Woon, Chua, Goh, Yang, Tay, Koh and Chiam. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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