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Title: Structure of a consensus chitin-binding domain revealed by solution NMR
Authors: Heymann, Dario
Mohanram, Harini
Kumar, Akshita
Verma, Chandra Shekhar
Lescar, Julien
Miserez, Ali
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Heymann, D., Mohanram, H., Kumar, A., Verma, C. S., Lescar, J. & Miserez, A. (2021). Structure of a consensus chitin-binding domain revealed by solution NMR. Journal of Structural Biology, 213(2), 107725-.
Project: MOE2015-T2-1-062
Journal: Journal of Structural Biology
Abstract: Chitin-binding proteins (CBPs) are a versatile group of proteins found in almost every organism on earth. CBPs are involved in enzymatic carbohydrate degradation and also serve as templating scaffolds in the exoskeleton of crustaceans and insects. One specific chitin-binding motif found across a wide range of arthropods' exoskeletons is the "extended Rebers and Riddiford" consensus (R&R), whose mechanism of chitin binding remains unclear. Here, we report the 3D structure and molecular level interactions of a chitin-binding domain (CBD-γ) located in a CBP from the beak of the jumbo squid Dosidicus gigas. This CBP is one of four chitin-binding proteins identified in the beak mouthpart of D. gigas and is believed to interact with chitin to form a scaffold network that is infiltrated with a second set of structural proteins during beak maturation. We used solution state NMR spectroscopy to elucidate the molecular interactions between CBD-γ and the soluble chitin derivative pentaacetyl-chitopentaose (PCP), and find that folding of CBD-γ is triggered upon its interaction with PCP. To our knowledge, this is the first experimental 3D structure of a CBP containing the R&R consensus motif, which can be used as a template to understand in more details the role of the R&R motif found in a wide range of CBP-chitin complexes. The present structure also provides molecular information for biomimetic synthesis of graded biomaterials using aqueous-based chemistry and biopolymers.
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2021.107725
Schools: School of Materials Science and Engineering 
School of Biological Sciences 
Organisations: Bioinformatics Institute, A*STAR
Research Centres: Biological & Biomimetic Material Laboratory @ NTU 
Center for Sustainable Materials
NTU Institute of Structural Biology 
Rights: © 2021 Elsevier Inc. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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