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Title: Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces
Authors: Meker, Sigalit
Halevi, Oded
Chin, Hokyun
Sut, Tun Naw
Jackman, Joshua A.
Tan, Ee-Lin
Potroz, Michael G.
Cho, Nam-Joon
Keywords: Engineering::Materials
Issue Date: 2022
Source: Meker, S., Halevi, O., Chin, H., Sut, T. N., Jackman, J. A., Tan, E., Potroz, M. G. & Cho, N. (2022). Inkjet-printed phospholipid bilayers on titanium oxide surfaces: towards functional membrane biointerfaces. Membranes, 12(4), 361-.
Project: 2020-T1-002-032 (RG111/20) 
Journal: Membranes 
Abstract: Functional biointerfaces hold broad significance for designing cell-responsive medical implants and sensor devices. Solid-supported phospholipid bilayers are a promising class of biological materials to build bioinspired thin-film coatings, as they can facilitate interactions with cell membranes. However, it remains challenging to fabricate lipid bilayers on medically relevant materials such as titanium oxide surfaces. There are also limitations in existing bilayer printing capabilities since most approaches are restricted to either deposition alone or to fixed microarray patterning. By combining advances in lipid surface chemistry and on-demand inkjet printing, we demonstrate the direct deposition and patterning of covalently tethered lipid bilayer membranes on titanium oxide surfaces, in ambient conditions and without any surface pretreatment process. The deposition conditions were evaluated by quartz crystal microbalance-dissipation (QCM-D) measurements, with corresponding resonance frequency (Δf) and energy dissipation (ΔD) shifts of around -25 Hz and <1 × 10-6, respectively, that indicated successful bilayer printing. The resulting printed phospholipid bilayers are stable in air and do not collapse following dehydration; through rehydration, the bilayers regain their functional properties, such as lateral mobility (>1 µm2/s diffusion coefficient), according to fluorescence recovery after photobleaching (FRAP) measurements. By taking advantage of the lipid bilayer patterned architectures and the unique features of titanium oxide's photoactivity, we further show how patterned cell culture arrays can be fabricated. Looking forward, this work presents new capabilities to achieve stable lipid bilayer patterns that can potentially be translated into implantable biomedical devices.
ISSN: 2077-0375
DOI: 10.3390/membranes12040361
Schools: School of Materials Science and Engineering 
Rights: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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