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Title: CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function
Authors: Rouers, Angeline
Appanna, Ramapraba
Chevrier, Marion
Lum, Josephine
Lau, Mai Chan
Tan, Lingqiao
Loy, Thomas
Tay, Alicia
Sethi, Raman
Sathiakumar, Durgalakshmi
Kaur, Kaval
Böhme, Julia
Leo, Yee-Sin
Renia, Laurent
Howland, Shanshan W
Singhal, Amit
Chen, Jinmiao
Fink, Katja
Keywords: Science::Medicine
Issue Date: 2021
Source: Rouers, A., Appanna, R., Chevrier, M., Lum, J., Lau, M. C., Tan, L., Loy, T., Tay, A., Sethi, R., Sathiakumar, D., Kaur, K., Böhme, J., Leo, Y., Renia, L., Howland, S. W., Singhal, A., Chen, J. & Fink, K. (2021). CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function. IScience, 24(5), 102482-.
Project: #H16/99/b0/011
Journal: iScience
Abstract: Clinically important broadly reactive B cells evolve during multiple infections, with B cells re-activated after secondary infection differing from B cells activated after a primary infection. Here we studied CD27highCD38high plasmablasts from patients with a primary or secondary dengue virus infection. Three transcriptionally and functionally distinct clusters were identified. The largest cluster 0/1 was plasma cell-related, with cells coding for serotype cross-reactive antibodies of the IgG1 isotype, consistent with memory B cell activation during an extrafollicular response. Cells in clusters 2 and 3 expressed low levels of antibody genes and high levels of genes associated with oxidative phosphorylation, EIF2 pathway, and mitochondrial dysfunction. Clusters 2 and 3 showed a transcriptional footprint of T cell help, in line with activation from naive B cells or memory B cells. Our results contribute to the understanding of the parallel B cell activation events that occur in humans after natural primary and secondary infection.
ISSN: 2589-0042
DOI: 10.1016/j.isci.2021.102482
Schools: School of Biological Sciences 
Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: National Centre for Infectious Diseases
Tan Tock Seng Hospital
National University of Singapore
Rights: © 2021 The Authors. This is an open access article under the CC BY-NC-ND license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles

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