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Title: Novel phage lysin Abp013 against Acinetobacter baumannii
Authors: Chu, Joash Jun Keat
Poh, Wee Han
Nabilah Taqiah Hasnuddin
Hew, En Yi
Dam, Linh Chi
El Sahili, Abbas
Rice, Scott A.
Goh, Boon Chong
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Chu, J. J. K., Poh, W. H., Nabilah Taqiah Hasnuddin, Hew, E. Y., Dam, L. C., El Sahili, A., Rice, S. A. & Goh, B. C. (2022). Novel phage lysin Abp013 against Acinetobacter baumannii. Antibiotics, 11(2), 169-.
Project: ITS-006-003 
Journal: Antibiotics 
Abstract: As antimicrobial resistance (AMR) continues to pose an ever-growing global health threat, propelling us into a post-antibiotic era, novel alternative therapeutic agents are urgently required. Lysins are bacteriophage-encoded peptidoglycan hydrolases that display great potential as a novel class of antimicrobials for therapeutics. While lysins against Gram-positive bacteria are highly effective when applied exogenously, it is challenging for lysins to access and cleave the peptidoglycan of Gram-negative bacteria due to their outer membrane. In this study, we identify a novel phage lysin Abp013 against Acinetobacter baumannii. Abp013 exhibited significant lytic activity against multidrug-resistant strains of A. baumannii. Notably, we found that Abp013 was able to tolerate the presence of human serum by up to 10%. Using confocal microscopy and LIVE/DEAD staining, we show that Abp013 can access and kill the bacterial cells residing in the biofilm. These results highlight the intrinsic bacteriolytic property of Abp013, suggesting the promising use of Abp013 as a novel therapeutic agent.
ISSN: 2079-6382
DOI: 10.3390/antibiotics11020169
Schools: School of Biological Sciences 
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) 
Rights: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
SCELSE Journal Articles

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