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|Title:||Genomic and phenotypic characterization of chloracidobacterium isolates provides evidence for multiple species||Authors:||Saini, Mohit Kumar
Soulier, Nathan T.
Costas, Amaya M. Garcia
Drautz-Moses, Daniela Isabel
Schuster, Stephan Christoph
Bryant, Donald A.
|Keywords:||Science::Biological sciences||Issue Date:||2021||Source:||Saini, M. K., Sebastian, A., Shirotori, Y., Soulier, N. T., Costas, A. M. G., Drautz-Moses, D. I., Schuster, S. C., Albert, I., Haruta, S., Hanada, S., Thiel, V., Tank, M. & Bryant, D. A. (2021). Genomic and phenotypic characterization of chloracidobacterium isolates provides evidence for multiple species. Frontiers in Microbiology, 12, 704168-. https://dx.doi.org/10.3389/fmicb.2021.704168||Journal:||Frontiers in Microbiology||Abstract:||Chloracidobacterium is the first and until now the sole genus in the phylum Acidobacteriota (formerly Acidobacteria) whose members perform chlorophyll-dependent phototrophy (i.e., chlorophototrophy). An axenic isolate of Chloracidobacterium thermophilum (strain B T ) was previously obtained by using the inferred genome sequence from an enrichment culture and diel metatranscriptomic profiling analyses in situ to direct adjustments to the growth medium and incubation conditions, and thereby a defined growth medium for Chloracidobacterium thermophilum was developed. These advances allowed eight additional strains of Chloracidobacterium spp. to be isolated from microbial mat samples collected from Mushroom Spring, Yellowstone National Park, United States, at temperatures of 41, 52, and 60°C; an axenic strain was also isolated from Rupite hot spring in Bulgaria. All isolates are obligately photoheterotrophic, microaerophilic, non-motile, thermophilic, rod-shaped bacteria. Chloracidobacterium spp. synthesize multiple types of (bacterio-)chlorophylls and have type-1 reaction centers like those of green sulfur bacteria. Light harvesting is accomplished by the bacteriochlorophyll a-binding, Fenna-Matthews-Olson protein and chlorosomes containing bacteriochlorophyll c. Their genomes are approximately 3.7 Mbp in size and comprise two circular chromosomes with sizes of approximately 2.7 Mbp and 1.0 Mbp. Comparative genomic studies and phenotypic properties indicate that the nine isolates represent three species within the genus Chloracidobacterium. In addition to C. thermophilum, the microbial mats at Mushroom Spring contain a second species, tentatively named Chloracidobacterium aggregatum, which grows as aggregates in liquid cultures. The Bulgarian isolate, tentatively named Chloracidobacterium validum, will be proposed as the type species of the genus, Chloracidobacterium. Additionally, Chloracidobacterium will be proposed as the type genus of a new family, Chloracidobacteriaceae, within the order Blastocatellales, the class Blastocatellia, and the phylum Acidobacteriota.||URI:||https://hdl.handle.net/10356/160740||ISSN:||1664-302X||DOI:||10.3389/fmicb.2021.704168||Research Centres:||Singapore Centre for Environmental Life Sciences and Engineering||Rights:||© 2021 Saini, Sebastian, Shirotori, Soulier, Garcia Costas, Drautz-Moses, Schuster, Albert, Haruta, Hanada, Thiel, Tank and Bryant. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
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