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dc.contributor.authorKumar, Ambrishen_US
dc.contributor.authorNandwana, Vikasen_US
dc.contributor.authorRyoo, Soo-Ryoonen_US
dc.contributor.authorRavishankar, Samyuktaen_US
dc.contributor.authorSharma, Bhargyen_US
dc.contributor.authorPervushin, Konstantinen_US
dc.contributor.authorDravid, Vinayak P.en_US
dc.contributor.authorLim, Sierinen_US
dc.identifier.citationKumar, A., Nandwana, V., Ryoo, S., Ravishankar, S., Sharma, B., Pervushin, K., Dravid, V. P. & Lim, S. (2021). Magnetoferritin enhances T₂ contrast in magnetic resonance imaging of macrophages. Materials Science and Engineering: C, 128, 112282-.
dc.description.abstractImaging of immune cells has wide implications in understanding disease progression and staging. While optical imaging is limited in penetration depth due to light properties, magnetic resonance (MR) imaging provides a more powerful tool for the imaging of deep tissues where immune cells reside. Due to poor MR signal to noise ratio, tracking of such cells typically requires contrast agents. This report presents an in-depth physical characterization and application of archaeal magnetoferritin for MR imaging of macrophages - an important component of the innate immune system that is the first line of defense and first responder in acute inflammation. Magnetoferritin is synthesized by loading iron in apoferritin in anaerobic condition at 65 °C. The loading method results in one order of magnitude enhancement of r1 and r2 relaxivities compared to standard ferritin synthesized by aerobic loading of iron at room temperature. Detailed characterizations of the magnetoferritin revealed a crystalline core structure that is distinct from previously reported ones indicating magnetite form. The magnetite core is more stable in the presence of reducing agents and has higher peroxidase-like activities compared to the core in standard loading. Co-incubation of macrophage cells with magnetoferritin in-vitro shows significantly higher enhancement in T2-MRI contrast of the immune cells compared to standard ferritin.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.relation.ispartofMaterials Science and Engineering: Cen_US
dc.rights© 2021 Elsevier B.V. All rights reserved.en_US
dc.titleMagnetoferritin enhances T₂ contrast in magnetic resonance imaging of macrophagesen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.researchNTU-Northwestern Institute for Nanomedicineen_US
dc.subject.keywordsProtein Cageen_US
dc.description.acknowledgementThis work was supported by NTU-Northwestern Institute for Nano-medicine located at the International Institute for Nanotechnology, Northwestern University, USA and the Nanyang Technological Univer-sity, Singapore (Grant No. M4081504.F40.706022).en_US
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